Citation: Ying Wen, Ning Jing, Fangjun Huo, Caixia Yin. Rational design of a turn-on fluorescent probe for visualization of GRP78 protein in tumor models[J]. Chinese Chemical Letters, ;2023, 34(3): 107604. doi: 10.1016/j.cclet.2022.06.027 shu

Rational design of a turn-on fluorescent probe for visualization of GRP78 protein in tumor models

Ying Wen ^a ,
Ning Jing ^a ,
Fangjun Huo ^b ,
Caixia Yin ^{a, *,}

a.
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
b.
Research Institute of Applied Chemistry, Shanxi University, Taiyuan 030006, China

yincx@sxu.edu.cn

Received Date: 8 March 2022
Revised Date: 8 June 2022
Accepted Date: 9 June 2022
Available Online: 14 June 2022

Figures(4)

Fluorescence image for accurate tumor label still faces challenges in cancer detection and diagnostics. Emerging evidence is indicating that glucose-regulated protein 78 (GRP78), a stress-inducible protein chaperone, is a great potential biomarker and therapeutic target for cancer. However, currently available probe for image tumor based on GRP78 has not been reported, owning to no obvious strategy in probe design towards this protein. In this paper, a hairpin-shaped peptidyl probe (pepFAM) conjugated with a 5-FAM fluorophore and a dabcyl quencher at both ends was developed, respectively. The probe was designed by performing a traditional fluorescence resonance energy transfer mechanism and employing a GRP78 specifically-binding peptide. Furthermore, the probe was used to specifically image cancer cells, and accurately image xenograft tumors in mice models. The novel fluorescent probe is expected to be a useful tool for the diagnostics of cancer.
- Glucose-regulated protein 78,
- Fluorescence probe,
- Living cell imaing,
- Tumor diagnosis
1. [1]
  M. Gao, F. Yu, C. Lv, J. Choo, L. Chen, Chem. Soc. Rev. 46 (2017) 2237-2271. doi: 10.1039/C6CS00908E
2. [2]
  K.I. Matsumoto, J.B. Mitchell, M.C. Krishna, Molecules 26 (2021), 1614. doi: 10.3390/molecules26061614
3. [3]
  S.H. Gardner, C.J. Reinhardt, J. Chan, Angew. Chem. Int. Ed. 60 (2021) 5000-5009. doi: 10.1002/anie.202003687
4. [4]
  J. Liu, M. Liu, H. Zhang, W. Guo, Angew. Chem. Int. Ed. 60 (2021) 12992-12998. doi: 10.1002/anie.202102377
5. [5]
  X. Zhang, Y. Chen, H. He, et al., Angew. Chem. Int. Ed. 60 (2021) 26337-26341. doi: 10.1002/anie.202109728
6. [6]
  Z. Zhao, C.B. Swartchick, J. Chan, Chem. Soc. Rev. 51 (2022) 829-868. doi: 10.1039/d0cs00771d
7. [7]
  L. Wu, X. Qu, Chem. Soc. Rev. 44 (2015) 2963-2997. doi: 10.1039/C4CS00370E
8. [8]
  Y. Wen, C.L. Schreiber, B.D. Smith, Bioconjug. Chem. 31 (2020) 474-482. doi: 10.1021/acs.bioconjchem.9b00836
9. [9]
  I. Hernandez, M. Cohen, Cancer Lett. 524 (2022) 1-14. doi: 10.1016/j.canlet.2021.10.004
10. [10]
  M. Wang, S. Wey, Y. Zhang, R. Ye, A.S. Lee, Antioxid. Redox Signal. 11 (2009) 2307-2316. doi: 10.1089/ars.2009.2485
11. [11]
  I.M. Ibrahim, D.H. Abdelmalek, A.A. Elfiky, Life Sci. 226 (2019) 156-163. doi: 10.1016/j.lfs.2019.04.022
12. [12]
  U. Gopal, S.V. Pizzo, J. Cell. Physiol. 236 (2021) 2352-2363. doi: 10.1002/jcp.30030
13. [13]
  M. Farshbaf, A.Y. Khosroushahi, S. Mojarad-Jabali, et al., J. Control. Release 328 (2020) 932-941. doi: 10.1016/j.jconrel.2020.10.055
14. [14]
  Y. Huang, Y. Zhang, F. Huo, et al., J. Am. Chem. Soc. 142 (2020) 18706-18714. doi: 10.1021/jacs.0c10210
15. [15]
  R.J. Passarella, D.E. Spratt, A.E. van der Ende, et al., Cancer Res. 70 (2010) 4550-4559. doi: 10.1158/0008-5472.CAN-10-0339
16. [16]
  M. Gonzalez-Gronow, U. Gopal, R.C. Austin, S.V. Pizzo, IUBMB Life 73 (2021) 843-854. doi: 10.1002/iub.2502
17. [17]
  S. Xia, W. Duan, W. Liu, X. Zhang, Q. Wang, J. Transl. Med. 19 (2021) 118. doi: 10.1186/s12967-021-02786-6
18. [18]
  Y.L. Tsai, A.S. Lee, Cell surface GRP78: anchoring and translocation mechanisms and therapeutic potential in cancer, in: S.V. Pizzo (Ed. ), Cell Surface GRP78, a New Paradigm in Signal Transduction Biology, Academic Press, New York, 2018, pp. 41-62.
19. [19]
  M.A. Arap, J. Lahdenranta, P.J. Mintz, et al., Cancer Cell 6 (2004) 275-284. doi: 10.1016/j.ccr.2004.08.018
20. [20]
  A.N.I. Viswanath, J.W. Lim, S.H. Seo, et al., Chem. Biol. Drug Des. 92 (2018) 1555-1566. doi: 10.1111/cbdd.13322
21. [21]
  Z. Li, C. Zhao, Z. Li, et al., Int. J. Biochem. Cell Biol. 47 (2014) 68-75. doi: 10.1016/j.biocel.2013.11.022
22. [22]
  P. Wei, L. Liu, Y. Wen, et al., Angew. Chem. Int. Ed. 58 (2019) 4547-4551. doi: 10.1002/anie.201813648
23. [23]
  X. Wu, R. Wang, N. Kwon, H. Ma, J. Yoon, Chem. Soc. Rev. 51 (2022) 450-463. doi: 10.1039/d1cs00543j
24. [24]
  Y.F. Huang, Y.B. Zhang, F.J. Huo, Y. Wen, C.X. Yin, Sci. China Chem. 63 (2020) 1742-1755. doi: 10.1007/s11426-020-9855-3
25. [25]
  L. Liu, F. Liu, D. Liu, et al., Angew. Chem. Int. Ed. 61 (2022) e202116807.
26. [26]
  W. Zhang, F. Huo, F. Cheng, C. Yin, J. Am. Chem. Soc. 142 (2020) 6324-6331. doi: 10.1021/jacs.0c00992
27. [27]
  Y. Wen, F. Huo, J. Wang, C. Yin, Anal. Chem. 91 (2019) 15057-15063. doi: 10.1021/acs.analchem.9b03731
28. [28]
  Y. Wen, F.J. Huo, C.X. Yin, Chin. Chem. Lett. 30 (2019) 1834-1842. doi: 10.1016/j.cclet.2019.07.006
29. [29]
  E. Pazos, O. Vazquez, J.L. Mascarenas, M.E. Vazquez, Chem. Soc. Rev. 38 (2009) 3348-3359. doi: 10.1039/b908546g
30. [30]
  Y. Wen, F. Xue, H. Lan, et al., Biosens. Bioelectron. 91 (2017) 115-121. doi: 10.1016/j.bios.2016.12.027
1. [1]
  Jingqi Xin , Shupeng Han , Meichen Zheng , Chenfeng Xu , Zhongxi Huang , Bin Wang , Changmin Yu , Feifei An , Yu Ren . A nitroreductase-responsive nanoprobe with homogeneous composition and high loading for preoperative non-invasive tumor imaging and intraoperative guidance. Chinese Chemical Letters, 2024, 35(7): 109165-. doi: 10.1016/j.cclet.2023.109165
2. [2]
  Haixian Ren , Yuting Du , Xiaojing Yang , Fangjun Huo , Le Zhang , Caixia Yin . Development of ESIPT-based specific fluorescent probes for bioactive species based on the protection-deprotection of the hydroxyl. Chinese Chemical Letters, 2025, 36(2): 109867-. doi: 10.1016/j.cclet.2024.109867
3. [3]
  Ziqin Li , Kai Hao , Longwei Xiang , Huayu Tian . Cationic covalent organic framework nanocarriers integrating both efficient gene silencing and real-time gene detection. Chinese Chemical Letters, 2025, 36(4): 109943-. doi: 10.1016/j.cclet.2024.109943
4. [4]
  Peide Zhu , Yangjia Liu , Yaoyao Tang , Siqi Zhu , Xinyang Liu , Lei Yin , Quan Liu , Zhiqiang Yu , Quan Xu , Dixian Luo , Juncheng Wang . Bi-doped carbon quantum dots functionalized liposomes with fluorescence visualization imaging for tumor diagnosis and treatment. Chinese Chemical Letters, 2024, 35(4): 108689-. doi: 10.1016/j.cclet.2023.108689
5. [5]
  Zhaorui Song , Qiulian Hao , Bing Li , Yuwei Yuan , Shanshan Zhang , Yongkuan Suo , Hai-Hao Han , Zhen Cheng . NIR-Ⅱ fluorescence lateral flow immunosensor based on efficient energy transfer probe for point-of-care testing of tumor biomarkers. Chinese Chemical Letters, 2025, 36(1): 109834-. doi: 10.1016/j.cclet.2024.109834
6. [6]
  Linfang ZHANG , Wenzhu YIN , Gui YIN . A 2-dicyanomethylene-3-cyano-4,5,5-trimethyl-2,5-dihydrofuran-based near-infrared fluorescence probe for the detection of hydrogen sulfide and imaging of living cells. Chinese Journal of Inorganic Chemistry, 2025, 41(3): 540-548. doi: 10.11862/CJIC.20240405
7. [7]
  Han-Min Wang , Yan-Chen Li , Lu-Lu Sun , Ming-Ye Tang , Jia Liu , Jiahao Cai , Lei Dong , Jia Li , Yi Zang , Hai-Hao Han , Xiao-Peng He . Protein-encapsulated long-wavelength fluorescent probe hybrid for imaging lipid droplets in living cells and mice with non-alcoholic fatty liver. Chinese Chemical Letters, 2024, 35(11): 109603-. doi: 10.1016/j.cclet.2024.109603
8. [8]
  Zhihui Zhang , Ru Sun , Chong Bian , Hongbo Wang , Zhen Zhao , Panpan Lv , Jianzhong Lu , Haixin Zhang , Hulie Zeng , Yuanyuan Chen , Zhijuan Cao . A dual-protease-triggered chemiluminescent probe for precise tumor imaging. Chinese Chemical Letters, 2025, 36(2): 109784-. doi: 10.1016/j.cclet.2024.109784
9. [9]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
10. [10]
  Zhixiao Xiong , Shanni Qiu , Yuyu Wang , Houna Duan , Yi Xiao , Yufang Xu , Weiping Zhu , Xuhong Qian . Photocalibrated NO release from the zinc ion fluorescent probe based on naphthalimide and its application in living cells. Chinese Chemical Letters, 2025, 36(4): 110002-. doi: 10.1016/j.cclet.2024.110002
11. [11]
  Hui Zhang , Rong Feng , Wanyi Yu , Hongbei Wei , Tianhong Wu , Peng Zhang , Wenhai Bian , Xin Li , Di Gao , Guojun Weng , Zhe Yang , Tony D. James , Xiaolong Sun . Evaluating the global thiols redox state in living cells using a reducing sulfur species responsive fluorescence switching platform. Chinese Chemical Letters, 2025, 36(4): 110528-. doi: 10.1016/j.cclet.2024.110528
12. [12]
  Biao Huang , Tao Tang , Fushou Liu , Shi-Hui Chen , Zhi-Ling Zhang , Mingxi Zhang , Ran Cui . Quantum dots boost large-view NIR-Ⅱ imaging with high fidelity for fluorescence-guided tumor surgery. Chinese Chemical Letters, 2024, 35(12): 109694-. doi: 10.1016/j.cclet.2024.109694
13. [13]
  Yudi Cheng , Xiao Wang , Jiao Chen , Zihan Zhang , Jiadong Ou , Mengyao She , Fulin Chen , Jianli Li . A near-infrared fluorescent probe for visualizing transformation pathway of Cys/Hcy and H₂S and its applications in living system. Chinese Chemical Letters, 2024, 35(5): 109156-. doi: 10.1016/j.cclet.2023.109156
14. [14]
  Ying Gao , Rong Zhou , Qiwen Wang , Shaolong Qi , Yuanyuan Lv , Shuang Liu , Jie Shen , Guocan Yu . Natural killer cell membrane doped supramolecular nanoplatform with immuno-modulatory functions for immuno-enhanced tumor phototherapy. Chinese Chemical Letters, 2024, 35(10): 109521-. doi: 10.1016/j.cclet.2024.109521
15. [15]
  Yuanzheng Wang , Chen Zhang , Shuyan Han , Xiaoli Kong , Changyun Quan , Jun Wu , Wei Zhang . Cancer cell membrane camouflaged biomimetic gelatin-based nanogel for tumor inhibition. Chinese Chemical Letters, 2024, 35(11): 109578-. doi: 10.1016/j.cclet.2024.109578
16. [16]
  Yanfei Liu , Yaqin Hu , Yifu Tan , Qiwen Chen , Zhenbao Liu . Tumor acidic microenvironment activatable DNA nanostructure for precise cancer cell targeting and inhibition. Chinese Chemical Letters, 2025, 36(1): 110289-. doi: 10.1016/j.cclet.2024.110289
17. [17]
  Qiuye Wang , Yabing Sun , Liangxue Lai , Haijing Cui , Yonglong Ye , Ming Yang , Weihao Zhu , Bo Yuan , Quanliang Mao , Wenzhi Ren , Aiguo Wu . MMP-9-responsive probe for fluorescence-magnetic resonance dual-mode imaging of hepatocellular carcinoma models with different metastatic capacities. Chinese Chemical Letters, 2025, 36(4): 110212-. doi: 10.1016/j.cclet.2024.110212
18. [18]
  Lixian Fu , Yiyun Tan , Yue Ding , Weixia Qing , Yong Wang . Water–soluble and polarity–sensitive near–infrared fluorescent probe for long–time specific cancer cell membranes imaging and C. Elegans label. Chinese Chemical Letters, 2024, 35(4): 108886-. doi: 10.1016/j.cclet.2023.108886
19. [19]
  Lei Shen , Hongmei Liu , Ming Jin , Jinchao Zhang , Caixia Yin , Shuxiang Wang , Yutao Yang . “Three-in-one” strategy of trifluoromethyl regulated blood-brain barrier permeable fluorescent probe for peroxynitrite and antiepileptic evaluation of edaravone. Chinese Chemical Letters, 2024, 35(10): 109572-. doi: 10.1016/j.cclet.2024.109572
20. [20]
  Kezuo Di , Jie Wei , Lijun Ding , Zhiying Shao , Junling Sha , Xilong Zhou , Huadong Heng , Xujing Feng , Kun Wang . A wearable sensor device based on screen-printed chip with biofuel cell-driven electrochromic display for noninvasive monitoring of glucose concentration. Chinese Chemical Letters, 2025, 36(2): 109911-. doi: 10.1016/j.cclet.2024.109911

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(828)
HTML views(59)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142