Citation: Xinqi Chang, Nina Wang, Depeng Jiang, Hong-Yuan Chen, Dechen Jiang. Nanokit coupled electrospray ionization mass spectrometry for analysis of angiotensin converting enzyme 2 activity in single living cell[J]. Chinese Chemical Letters, ;2023, 34(3): 107522. doi: 10.1016/j.cclet.2022.05.036 shu

Nanokit coupled electrospray ionization mass spectrometry for analysis of angiotensin converting enzyme 2 activity in single living cell

Xinqi Chang ^a ,
Nina Wang ^a ,
Depeng Jiang ^{b, *,} ,
Hong-Yuan Chen ^a ,
Dechen Jiang ^{a, **,}

a.
State Key Laboratory of Analytical Chemistry for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China
b.
Department of Respiratory Medicine, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, China

gdp116@163.com

dechenjiang@nju.edu.cn

Received Date: 12 January 2022
Revised Date: 20 April 2022
Accepted Date: 12 May 2022
Available Online: 16 May 2022

Figures(4)

Angiotensin-converting enzyme 2 (ACE2) is not only an enzyme but also a functional receptor on cell membrane for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Here, the activity of ACE2 in single living cell is firstly determined using a nanokit coupled electrospray ionization mass spectrometry (nanokit-ESI-MS). Upon the insertion of a micro-capillary into the living hACE2-CHO cell and the electrochemical sorting of the cytosol, the target ACE2 enzyme hydrolyses angiotensin II inside the capillary to generate angiotensin 1–7. After the electrospray of the mixture at the tip of the capillary, the product is differentiated from the substrate in molecular weight to achieve the detection of ACE2 activity in single cells. The further measurement illustrates that the inflammatory state of cells does not lead to the significant change of ACE2 catalytic activity, which elucidates the relationship between intracellular ACE2 activity and inflammation at single cell level. The established strategy will provide a specific analytical method for further studying the role of ACE2 in the process of virus infection, and extend the application of nanokit based single cell analysis.
- Nanokit,
- Electrospray ionization mass spectrometry,
- Single cell analysis,
- The enzymatic activity,
- ACE2
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