Citation: Fei Xue, Jeroen J.L.M. Cornelissen, Quan Yuan, Shuqin Cao. Delivery of MicroRNAs by plant virus-based nanoparticles to functionally alter the osteogenic differentiation of human mesenchymal stem cells[J]. Chinese Chemical Letters, ;2023, 34(1): 107448. doi: 10.1016/j.cclet.2022.04.046 shu

Delivery of MicroRNAs by plant virus-based nanoparticles to functionally alter the osteogenic differentiation of human mesenchymal stem cells

Fei Xue ^a ,
Jeroen J.L.M. Cornelissen ^b ,
Quan Yuan ^{a, *,} ,
Shuqin Cao ^{a, *,}

a.
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, China
b.
Department of Molecules & Materials, MESA+ Institute for Nanotechnology, University of Twente, Enschede AE 7500, the Netherlands

yuanquan@scu.edu.cn

caoshuqin2020scu@foxmail.com

Received Date: 2 December 2021
Revised Date: 15 April 2022
Accepted Date: 19 April 2022
Available Online: 22 April 2022

Figures(5)

MicroRNA-26a (miR-26a) has been verified to promote osteogenic differentiation of mesenchymal stem cells in recent years. The main obstacles to its application in bone regeneration are instability in the physiological environment and low efficiency of cellular membrane penetration. To overcome these problems, we constructed a novel plant virus gene delivery system based on Cowpea chlorotic mottle virus (CCMV). By encapsulating miR-26a with purified capsid protein (CP) dimers derived from CCMV, CP-miR-26a (CP26a) virus-like particles (VLPs) were obtained. CP26a retained a structure similar to the native CCMV and protected miR-26a from digestion with its exterior CP. Moreover, CP26a featured similar cellular uptake efficiency, osteogenesis promotion ability, and better biocompatibility compared with Lipofectamine2000-miR-26a (lipo26a), which indicated a promising prospect for CCMV as a novel gene delivery system.
- VLPs,
- Cowpea chlorotic mottle virus,
- Gene delivery,
- MicroRNAs,
- Osteogenesis
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