Citation: Keda Chen, Xiaoxin Wu, Qingjing Wang, Ying Wang, Haiyan Zhang, Shuai Zhao, Chaonan Li, Zhiwen Hu, Zhimou Yang, Lanjuan Li. The protective effects of a d-tetra-peptide hydrogel adjuvant vaccine against H7N9 influenza virus in mice[J]. Chinese Chemical Letters, ;2023, 34(1): 107446. doi: 10.1016/j.cclet.2022.04.044 shu

The protective effects of a d-tetra-peptide hydrogel adjuvant vaccine against H7N9 influenza virus in mice

Keda Chen ^{a, b, 1} ,
Xiaoxin Wu ^{b, 1} ,
Qingjing Wang ^a ,
Ying Wang ^a ,
Haiyan Zhang ^c ,
Shuai Zhao ^b ,
Chaonan Li ^a ,
Zhiwen Hu ^{d, e} ,
Zhimou Yang ^{d, e, 1,} ,
Lanjuan Li ^{b, *,}

a.
Shulan International Medical College, Zhejiang Shuren University, Hangzhou 310015, China
b.
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
c.
Zhejiang Shuren College, Zhejiang Chinese Medical University, Hangzhou 310053, China
d.
State Key Laboratory of Medicinal Chemical Biology, Key Laboratory of Bioactive Materials, Ministry of Education, College of Life Sciences, Synergetic Innovation Center of Chemical Science and Engineering, and National Institute of Functional Materials, Nankai University, Tianjin 300071, China
e.
Jiangsu Center for the Collaboration and Innovation of Cancer Biotherapy, Cancer Institute, Xuzhou Medical University, Xuzhou 221004, China

yangzm@nankai.edu.cn

ljli@zju.edu.cn

Received Date: 23 December 2021
Revised Date: 9 April 2022
Accepted Date: 19 April 2022
Available Online: 22 April 2022

Figures(4)

Repeated waves of influenza virus H7N9 epidemics after 2013 have caused severe influenza in humans, with mortality reaching approximately 40%–50%. To prevent possible pandemics, the development of highly effective vaccines against influenza virus H7N9 is highly desired. In the present study, by taking advantage of the d-tetra-peptide adjuvant (G^DF^DF^DY), we reported a simple method to prepare H7N9 vaccines. Naproxen (Npx), with good anti inflammatory and broad anti-viral effects, was employed as an N-terminal capping group to construct a hydrogel precursor, Npx-G^DF^DF^DY. The hydrogel adjuvant was prepared using a routine heating cooling protocol and the final vaccine was ready after mixing with the split A/Zhejiang/DTID-ZJU01/2013 (H7N9) antigen by vortexing. Compared with the traditional Al(OH)₃ adjuvant vaccine and the split vaccine, our hydrogel adjuvant vaccine showed the best preventive effects against H7N9 infection. A mechanistic study illustrated that higher antibody responses and variations in cytokine expression might account for its increased protective effects. Our strategy demonstrated the advantages of a peptide hydrogel adjuvant in the application of vaccines against H7N9 and demonstrated its potential application in vaccines against emerging threats from other viruses.
- H7N9 influenza virus,
- Hydrogel,
- Adjuvant,
- Vaccine,
- Preventative effects
1. [1]
  G.I. Collaborators, Lancet Respir. Med. 7 (2019) 69–89. doi: 10.1016/S2213-2600(18)30496-X
2. [2]
  Y. Chen, W. Liang, S. Yang, et al., Lancet 381 (2013) 1916–1925. doi: 10.1016/S0140-6736(13)60903-4
3. [3]
  H.N. Gao, H.Z. Lu, B. Cao, et al., N. Engl. J. Med. 368 (2013) 2277–2285. doi: 10.1056/NEJMoa1305584
4. [4]
  J. Guo, F. Huang, J. Liu, et al., Sci. Rep. 5 (2015) 10942. doi: 10.1038/srep10942
5. [5]
  S. Zheng, Q. Zou, X. Wang, et al., Clin. Infect. Dis. 71 (2020) 128–132. doi: 10.1093/cid/ciz779
6. [6]
  C. Gao, Y. Wang, X. Gu, et al., Community Acquir. Pneumonia China Crit. Care Med. 48 (2020) 451–458.
7. [7]
  X.X. Wu, H.N. Gao, H.B. Wu, et al., Int. J. Infect. Dis. 41 (2015) 3–5. doi: 10.1016/j.ijid.2015.10.009
8. [8]
  B. Cao, H. Gao, B. Zhou, et al., Crit. Care Med. 44 (2016) e318–e328. doi: 10.1097/CCM.0000000000001616
9. [9]
  Y. Zhang, H. Gao, W. Liang, et al., BMC Infect. Dis. 16 (2016) 76.
10. [10]
  X. Wang, H. Jiang, P. Wu, et al., Lancet Infect. Dis. 17 (2017) 822–832. doi: 10.1016/S1473-3099(17)30323-7
11. [11]
  S. Zheng, L. Tang, H. Gao, et al., Clin. Infect. Dis. 66 (2018) 1054–1060. doi: 10.1093/cid/cix930
12. [12]
  X. Wu, L. Xiao, L. Li, Front. Med. 14 (2020) 8–20. doi: 10.1007/s11684-020-0739-z
13. [13]
  X.X. Wu, L.Z. Zhao, S.J. Tang, et al., Emerg. Microbes Infect. 9 (2020) 962–975. doi: 10.1080/22221751.2020.1754135
14. [14]
  T. Qin, S. Ma, X. Miao, et al., Adv. Sci. 7 (2020) 2000771. doi: 10.1002/advs.202000771
15. [15]
  H. Ou, H. Yao, W. Yao, et al., Vaccine 34 (2016) 2362–2370. doi: 10.1016/j.vaccine.2016.03.037
16. [16]
  H. Ou, W. Yao, N. Wu, et al., Oncotarget 7 (2016) 81012–81025. doi: 10.18632/oncotarget.12746
17. [17]
  H. Ou, W. Yao, D. Yu, et al., Oncotarget 8 (2017) 91828–91840. doi: 10.18632/oncotarget.20064
18. [18]
  X.X. Wu, X.L. Deng, D.S. Yu, et al., Cell Physiol. Biochem. 46 (2018) 633–643. doi: 10.1159/000488631
19. [19]
  Z. Yang, G. Liang, B. Xu, Soft Matter 3 (2007) 515–520. doi: 10.1039/b700138j
20. [20]
  Z. Yang, G. Liang, B. Xu, Acc. Chem. Res. 41 (2008) 315–326. doi: 10.1021/ar7001914
21. [21]
  A.N. Shy, B.J. Kim, B. Xu, Matte 1 (2019) 1127–1147. doi: 10.1016/j.matt.2019.09.015
22. [22]
  H.J. He, W.Y. Tan, J.Q. Guo, et al., Chem. Rev. 120 (2020) 9994–10078. doi: 10.1021/acs.chemrev.0c00306
23. [23]
  X.X. Li, Y.H. Wang, Y.M. Zhang, et al., Adv. Funct. Mater. 31 (2021) 2100729. doi: 10.1002/adfm.202100729
24. [24]
  C. Yang, F. Hu, X. Zhang, et al., Biomaterials 244 (2020) 119972. doi: 10.1016/j.biomaterials.2020.119972
25. [25]
  Y. Tian, H.M. Wang, Y. Liu, et al., Nano Lett. 14 (2014) 1439–1445. doi: 10.1021/nl404560v
26. [26]
  H.M. Wang, Z. Luo, Y.C.Z. Wang, et al., Adv. Funct. Mater. 26 (2016) 1822–1829. doi: 10.1002/adfm.201505188
27. [27]
  Z. Luo, Q. Wu, C. Yang, et al., Adv. Mater. 29 (2017) 1601776. doi: 10.1002/adma.201601776
28. [28]
  X.X. Li, Y.Z. Wang, S.W. Wang, et al., Nanoscale 12 (2020) 2111–2117. doi: 10.1039/C9NR08916K
29. [29]
  Y. Wang, X. Li, Y. Zhang, L. Wang, Z. Yang, Chem. Commun. 55 (2019) 12388–12391. doi: 10.1039/C9CC05633E
30. [30]
  J.S. Rudra, Y.F. Tian, J.P. Jung, J.H. Collier, Proc. Natl. Acad. Sci. 107 (2010) 622–627. doi: 10.1073/pnas.0912124107
31. [31]
  J. Chen, R.R. Pompano, F.W. Santiago, et al., Biomaterials 34 (2013) 8776–8785. doi: 10.1016/j.biomaterials.2013.07.063
32. [32]
  Z. Wang, C. Liang, F. Shi, et al., Nanoscale 9 (2017) 14058–14064. doi: 10.1039/C7NR04990K
33. [33]
  Y.M. Zhang, X.X. Li, D.B. Zheng, J. Gao, Z. Yang, Chin. Chem. Lett. 32 (2021) 3636–3640. doi: 10.1016/j.cclet.2021.04.039
34. [34]
  J.J. Chen, C.X. Hu, L.J. Chen, et al., Eng. PRC 6 (2020) 1153–1161. doi: 10.1016/j.eng.2020.02.006
35. [35]
  C.A. Russell, T.C. Jones, I.G. Barr, Vaccine 26 (2008) D31–D34. doi: 10.1016/j.vaccine.2008.07.078
36. [36]
  T.K. Tsang, S. Cauchemez, R.A. Perera, et al., J. Infect. Dis. 210 (2014) 684–692. doi: 10.1093/infdis/jiu186
37. [37]
  R.W. Frenck, N.P. Klein, N. Kitchin, et al., N. Engl. J. Med. 385 (2021) 239–250. doi: 10.1056/NEJMoa2107456
38. [38]
  Y. Wang, Y. Xie, J. Luo, et al., Nano Today 38 (2021) 101139. doi: 10.1016/j.nantod.2021.101139
39. [39]
  S. Tang, Z. Liu, W. Xu, et al., Nano Lett. 19 (2019) 5469–5475. doi: 10.1021/acs.nanolett.9b01974
40. [40]
  Y.H. Liu, M.J. Li, M.H. Zhu, et al., Chin. Chem. Lett. 32 (2021) 1963–1966. doi: 10.1016/j.cclet.2021.01.035
41. [41]
  X. Li, A.M. Aldayel, Z. Cui, J. Control. Release 173 (2014) 148–157. doi: 10.1016/j.jconrel.2013.10.032
42. [42]
  H. Wang, Z. Yang, Nanoscale 4 (2012) 5259–5267. doi: 10.1039/c2nr31149f
43. [43]
  H. Wang, Z. Feng, Y. Wang, et al., J. Am. Chem. Soc. 138 (2016) 16046–16055. doi: 10.1021/jacs.6b09783
44. [44]
  Y. Cai, H. Shen, J. Zhan, et al., J. Am. Chem. Soc. 139 (2017) 2876–2879. doi: 10.1021/jacs.6b12322
45. [45]
  C.H. Ren, Z.Y. Wang, X.L. Zhang, et al., Chem. Eng. J. 405 (2021) 127008. doi: 10.1016/j.cej.2020.127008
46. [46]
  R.S. Zhang, J.H. Zhou, D. Lin, et al., Chem. Eng. J. 421 (2021) 129623. doi: 10.1016/j.cej.2021.129623
47. [47]
  T.M. Strutt, K.K. McKinstry, Y. Kuang, et al., J. Immunol. 197 (2016) 3260–3270. doi: 10.4049/jimmunol.1600033
48. [48]
  T. Nakayama, T. Kumagai, Y. Kashiwagi, et al., Hum. Vaccin. Immunother. 14 (2018) 2990–2998. doi: 10.1080/21645515.2018.1498435
49. [49]
  D.A.G. Skibinski, L.A. Jones, Y.O. Zhu, et al., Sci. Rep. 8 (2018) 18007. doi: 10.1038/s41598-018-36703-7
50. [50]
  H.M. El Sahly, G. Makedonas, D. Corry, et al., Hum. Vaccin. Immunother. 16 (2020) 3138–3145. doi: 10.1080/21645515.2020.1750910
1. [1]
  Mengchen Liu , Yufei Zhang , Yi Xiao , Yang Wei , Meichen Bi , Huaide Jiang , Yan Yu , Shenghong Zhong . High stretchability and toughness of liquid metal reinforced conductive biocompatible hydrogels for flexible strain sensors. Chinese Journal of Structural Chemistry, 2025, 44(3): 100518-100518. doi: 10.1016/j.cjsc.2025.100518
2. [2]
  Xi Chen , Xue Zhang , Shuai Yang , Jie Wang , Tian Tang , Maling Gou . An adhesive hydrogel for the treatment of oral ulcers. Chinese Chemical Letters, 2025, 36(3): 110021-. doi: 10.1016/j.cclet.2024.110021
3. [3]
  Ningyue Xu , Jun Wang , Lei Liu , Changyang Gong . Injectable hydrogel-based drug delivery systems for enhancing the efficacy of radiation therapy: A review of recent advances. Chinese Chemical Letters, 2024, 35(8): 109225-. doi: 10.1016/j.cclet.2023.109225
4. [4]
  Tong Zhang , Xiaojing Liang , Licheng Wang , Shuai Wang , Xiaoxiao Liu , Yong Guo . An ionic liquid assisted hydrogel functionalized silica stationary phase for mixed-mode liquid chromatography. Chinese Chemical Letters, 2025, 36(1): 109889-. doi: 10.1016/j.cclet.2024.109889
5. [5]
  Ningning Gao , Yue Zhang , Zhenhao Yang , Lijing Xu , Kongyin Zhao , Qingping Xin , Junkui Gao , Junjun Shi , Jin Zhong , Huiguo Wang . Ba²⁺/Ca²⁺ co-crosslinked alginate hydrogel filtration membrane with high strength, high flux and stability for dye/salt separation. Chinese Chemical Letters, 2024, 35(5): 108820-. doi: 10.1016/j.cclet.2023.108820
6. [6]
  Yang Xu , Le Ma , Yang Wang , Chunmeng Shi . Engineering strategies of biomaterial-assisted exosomes for skin wound repair: Latest advances and challenges. Chinese Chemical Letters, 2025, 36(1): 109766-. doi: 10.1016/j.cclet.2024.109766
7. [7]
  Yue Sun , Yingnan Zhu , Jiahang Si , Ruikang Zhang , Yalan Ji , Jinjie Fan , Yuze Dong . Glucose-activated nanozyme hydrogels for microenvironment modulation via cascade reaction in diabetic wound. Chinese Chemical Letters, 2025, 36(4): 110012-. doi: 10.1016/j.cclet.2024.110012
8. [8]
  Yuxin Tian , Mengjun Li , Yang Yang , Chunhui Li , Yun Peng , Haiyin Yang , Mengyuan Zhao , Pengfei Wu , Shaobo Ruan , Yuanyu Huang , Chenguang Shen , Minghui Yang . An MPXV mRNA-LNP vaccine candidate elicits protective immune responses against monkeypox virus. Chinese Chemical Letters, 2024, 35(8): 109270-. doi: 10.1016/j.cclet.2023.109270
9. [9]
  Xin Li , Xuan Ding , Junkun Zhou , Hui Shi , Zhenxi Dai , Jiayi Liu , Yongcun Ma , Penghui Shao , Liming Yang , Xubiao Luo . Utilizing synergistic effects of bifunctional polymer hydrogel PAM-PAMPS for selective capture of Pb(Ⅱ) from wastewater. Chinese Chemical Letters, 2024, 35(7): 109158-. doi: 10.1016/j.cclet.2023.109158
10. [10]
  Qiang Zhou , Pingping Zhu , Wei Shao , Wanqun Hu , Xuan Lei , Haiyang Yang . Innovative Experimental Teaching Design for 3D Printing High-Strength Hydrogel Experiments. University Chemistry, 2024, 39(6): 264-270. doi: 10.3866/PKU.DXHX202310064
11. [11]
  Qingyang Cui , Feng Yu , Zirun Wang , Bangkun Jin , Wanqun Hu , Wan Li . From Jelly to Soft Matter: Preparation and Properties-Exploring of Different Kinds of Hydrogels. University Chemistry, 2024, 39(9): 338-348. doi: 10.3866/PKU.DXHX202309046
12. [12]
  Xiaoliu Liang , Chunliu Huang , Hui Liu , Hu Chen , Jiabao Shou , Hongwei Cheng , Gang Liu . Natural hydrogel dressings in wound care: Design, advances, and perspectives. Chinese Chemical Letters, 2024, 35(10): 109442-. doi: 10.1016/j.cclet.2023.109442
13. [13]
  Guilong Li , Wenbo Ma , Jialing Zhou , Caiqin Wu , Chenling Yao , Huan Zeng , Jian Wang . A composite hydrogel with porous and homogeneous structure for efficient osmotic energy conversion. Chinese Chemical Letters, 2025, 36(2): 110449-. doi: 10.1016/j.cclet.2024.110449
14. [14]
  Jinpeng Du , Junlin Chen , Yulong Shan , Tongliang Zhang , Yu Sun , Zhongqi Liu , Xiaoyan Shi , Wenpo Shan , Yunbo Yu , Hong He . Insight into the effects of C₃H₆ on fresh and hydrothermally aged Cu-SSZ-39 catalysts. Chinese Chemical Letters, 2025, 36(3): 110019-. doi: 10.1016/j.cclet.2024.110019
15. [15]
  Jianye Kang , Xinyu Yang , Xuhao Yang , Jiahui Sun , Yuhang Liu , Shutao Wang , Wenlong Song . Carbon dots-enhanced pH-responsive lubricating hydrogel based on reversible dynamic covalent bondings. Chinese Chemical Letters, 2024, 35(5): 109297-. doi: 10.1016/j.cclet.2023.109297
16. [16]
  Xinyue Lan , Junguang Liang , Churan Wen , Xiaolong Quan , Huimin Lin , Qinqin Xu , Peixian Chen , Guangyu Yao , Dan Zhou , Meng Yu . Photo-manipulated polyunsaturated fatty acid-doped liposomal hydrogel for flexible photoimmunotherapy. Chinese Chemical Letters, 2024, 35(4): 108616-. doi: 10.1016/j.cclet.2023.108616
17. [17]
  Zheyi Li , Xiaoyang Liang , Zitong Qiu , Zimeng Liu , Siyu Wang , Yue Zhou , Nan Li . Ion-interferential cell cycle arrest for melanoma treatment based on magnetocaloric bimetallic-ion sustained release hydrogel. Chinese Chemical Letters, 2024, 35(11): 109592-. doi: 10.1016/j.cclet.2024.109592
18. [18]
  Zhibin Ren , Shan Li , Xiaoying Liu , Guanghao Lv , Lei Chen , Jingli Wang , Xingyi Li , Jiaqing Wang . Penetrating efficiency of supramolecular hydrogel eye drops: Electrostatic interaction surpasses ligand-receptor interaction. Chinese Chemical Letters, 2024, 35(11): 109629-. doi: 10.1016/j.cclet.2024.109629
19. [19]
  Haijun Shen , Yi Qiao , Chun Zhang , Yane Ma , Jialing Chen , Yingying Cao , Wenna Zheng . A matrix metalloproteinase-sensitive hydrogel combined with photothermal therapy for transdermal delivery of deferoxamine to accelerate diabetic pressure ulcer healing. Chinese Chemical Letters, 2024, 35(12): 110283-. doi: 10.1016/j.cclet.2024.110283
20. [20]
  Jiajia Wang , XinXin Ge , Yajing Xiang , Xiaoliang Qi , Ying Li , Hangbin Xu , Erya Cai , Chaofan Zhang , Yulong Lan , Xiaojing Chen , Yizuo Shi , Zhangping Li , Jianliang Shen . An ionic liquid functionalized sericin hydrogel for drug-resistant bacteria-infected diabetic wound healing. Chinese Chemical Letters, 2025, 36(2): 109819-. doi: 10.1016/j.cclet.2024.109819

Get Citation

PDF

XML

Metrics

PDF Downloads(4)
Abstract views(825)
HTML views(66)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142