Citation: Fengjiao Zhu, Yahui Ji, Jiu Deng, Linmei Li, Xue Bai, Xianming Liu, Bingcheng Lin, Yao Lu. Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level[J]. Chinese Chemical Letters, ;2022, 33(6): 2893-2900. doi: 10.1016/j.cclet.2021.09.058 shu

Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

Fengjiao Zhu ^{a, b} ,
Yahui Ji ^a ,
Jiu Deng ^a ,
Linmei Li ^a ,
Xue Bai ^a ,
Xianming Liu ^a ,
Bingcheng Lin ^a ,
Yao Lu ^{a, *,}

a.
Department of Biotechnology, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

luyao@dicp.ac.cn

Received Date: 6 June 2021
Revised Date: 5 September 2021
Accepted Date: 17 September 2021
Available Online: 24 September 2021

Figures(6)

Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in mediating intercellular communications for various physiological and pathological processes. Most of the EV analysis is currently performed at the bulk level, obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle. Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion. Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims. Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis, which would be summarized in this mini-review.
- Microfluidics,
- Single-cell EV analysis,
- Single EV analysis,
- Extracellular vesicle,
- Heterogeneity
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