Citation: Longjie Li, Kejun Dong, Xinyu Wang, Meizhou Zhang, Jun Li, Andreas K. Nussler, Xianjin Xiao, Hongbo Wang, Yuzhou Wu. DNA origami-based nano-hunter enriches low-abundance point mutations by targeting wild-type gene segments[J]. Chinese Chemical Letters, ;2022, 33(4): 2052-2056. doi: 10.1016/j.cclet.2021.09.055 shu

DNA origami-based nano-hunter enriches low-abundance point mutations by targeting wild-type gene segments

Longjie Li ^{a, b, 1,} ,
Kejun Dong ^{c, d, 1} ,
Xinyu Wang ^e ,
Meizhou Zhang ^b ,
Jun Li ^b ,
Andreas K. Nussler ^f ,
Xianjin Xiao ^d ,
Hongbo Wang ^{c, **,} ,
Yuzhou Wu ^{b, **,}

a.
School of Life Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China
b.
Hubei Key Laboratory of Bioinorganic Chemistry and Materia Medica, Hubei Engineering Research Center for Biomaterials and Medical Protective Materials, School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Wuhan 430074, China
c.
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
d.
Institute of Reproductive Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China
e.
Department of Breast Surgery, Second Hospital of Jilin University, Changchun 130041, China
f.
Siegfried Weller Institute for Trauma Research, Eberhard-Karls-Universität Tübingen, Schnarrenbergstr. 95, D-72076, Tübingen, Germany

^**

lilongjie@whpu.edu.cn

hb_wang1969@sina.com

wuyuzhou@hust.edu.cn

Received Date: 7 August 2021
Revised Date: 3 September 2021
Accepted Date: 15 September 2021
Available Online: 21 September 2021

Figures(6)

Point mutations can be used as biomarkers to perform diagnosis for diseases. In this study, a nanorobot for low-abundance point mutation enrichment was constructed using DNA origami. The novel design achieved limits of detection of 0.1% and 1% for synthesized DNA samples and clinical gene samples, respectively. Resettability was a key property of this method, which also involved a simpler process, lower cost and shorter detection duration than traditional enrichment methods. This novel DNA nanorobot may enable the detection of tumor markers, potentially facilitating early cancer diagnosis.
- Low-abundance point mutation,
- Cancer diagnosis,
- DNA nano-hunter,
- Enrichment,
- Resettability
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