Citation: Xiaoting Huo, Lei Wang, Wuzhen Qi, Na Rong, Yingjia Liu, Siyuan Wang, Hong Duan, Jianhan Lin. A lab-on-a-disc platform based on nickel nanowire net and smartphone imaging for rapid and automatic detection of foodborne bacteria[J]. Chinese Chemical Letters, ;2022, 33(4): 2091-2095. doi: 10.1016/j.cclet.2021.08.027 shu

A lab-on-a-disc platform based on nickel nanowire net and smartphone imaging for rapid and automatic detection of foodborne bacteria

Xiaoting Huo ^{a, 1} ,
Lei Wang ^{a, 1} ,
Wuzhen Qi ^b ,
Na Rong ^b ,
Yingjia Liu ^a ,
Siyuan Wang ^b ,
Hong Duan ^b ,
Jianhan Lin ^{a, b, *,}

a.
Key Laboratory of Agricultural Information Acquisition Technology, Ministry of Agriculture and Rural Affairs, China Agricultural University, Beijing 100083, China
b.
Key Laboratory of Modern Precision Agriculture System Integration Research, Ministry of Education, China Agricultural University, Beijing 100083, China

jianhan@cau.edu.cn

Received Date: 31 May 2021
Revised Date: 4 August 2021
Accepted Date: 6 August 2021
Available Online: 12 August 2021

Figures(4)

Foodborne pathogenic bacteria have been considered as a major risk factor for food safety. It is of great significance to carry out in-field screening of pathogenic bacteria to prevent the outbreaks of foodborne diseases. In this study, a portable lab-on-a-disc platform with a microfluidic disc was developed for rapid and automatic detection of Salmonella typhimurium using a nickel nanowire (NiNW) net for effective separation of target bacteria, horseradish peroxidase nanoflowers (HRP NFs) for efficient amplification of biological signals, and a self-developed smartphone APP for accurate analysis of colorimetric images. First, the microfluidic disc was preloaded with reagents and samples and centrifuged to form one bacterial sample column, one immune NiNW column, one HRP NF column, two washing buffer columns and one tetramethylbenzidine (TMB) column, which were separated by air gaps. Then, a rotatable magnetic field was specifically developed to assemble the NiNWs into a net, which was automatically controlled by a stepped motor to successively pass through the sample column for specific capture of target bacteria, the HRP NF column for specific label of target bacteria, the washing columns for effective removal of sample background and non-specific binding NFs, and the TMB column for colorimetric determination of target bacteria. The color change of TMB from colorless to blue was finally analyzed using the smartphone APP to quantitatively determine the target bacteria. This lab-on-a-disc platform could detect Salmonella typhimurium from 5.6×10¹ CFU/20 μL to 5.6×10⁵ CFU/20 μL in 1 h with a lower detection limit of 56 CFU/20 μL. The recovery of target bacteria in spiked chicken samples ranged from 97.5% to 101.8%. This portable platform integrating separation, labeling, washing, catalysis and detection onto a single disc is featured with automatic operation, fast reaction, and small size and has shown its potential for in-field detection of foodborne pathogens.
- Lab-on-a-disk platform,
- Nickel nanowire net,
- Horseradish peroxidase nanoflowers,
- Smartphone,
- Bacteria detection
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