Citation: Xing Wei, Meng Yang, Ze Jiang, Jinhui Liu, Xuan Zhang, Mingli Chen, Jianhua Wang. A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis[J]. Chinese Chemical Letters, ;2022, 33(3): 1373-1376. doi: 10.1016/j.cclet.2021.08.024 shu

A modular single-cell pipette microfluidic chip coupling to ETAAS and ICP-MS for single cell analysis

Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang 110819, China

chenml@mail.neu.edu.cn

jianhuajrz@mail.neu.edu.cn

Received Date: 28 May 2021
Revised Date: 11 July 2021
Accepted Date: 6 August 2021
Available Online: 12 August 2021

Figures(4)

Accurate single-cell capture is a crucial step for single cell biological and chemical analysis. Conventional single-cell capturing often confront operational complexity, limited efficiency, cell damage, large scale but low accuracy, incompetence in the acquirement of nano-upgraded single-cell liquid. Flow cytometry has been widely used in large-scale single-cell detection, while precise single-cell isolation relies on both a precision operating platform and a microscope, which is not only extremely inefficient, but also not conducive to couple with modern analytical instruments. Herein, we develop a modular single-cell pipette (mSCP) microfluidic chip with high efficiency and strong applicability for accurate direct capture of single viable cell from cell suspensions into nanoliter droplets (30-1000 nL). The mSCP is used as a sampling platform for the detection of CdTe quantum dots in single cells with electrothermal atomic absorption spectrometry (ETAAS) for the first time. It also ensures precise single-cell sampling and detection by inductively coupled plasma mass spectrometry (ICP-MS).
- Single-cell pipette,
- Microfluidic chip,
- Single-cell capture,
- ETAAS,
- ICP-MS,
- CdTe QDs
1. [1]
  V. Almendro, A. Marusyk, K. Polyak, Annu. Rev. Pathol. Mech. Dis. 8(2013) 277-302. doi: 10.1146/annurev-pathol-020712-163923
2. [2]
  K. Zhang, X. Han, Y. Li, et al., J. Am. Chem. Soc. 136(2014) 10858-10861. doi: 10.1021/ja5053279
3. [3]
  C.E. Meacham, S.J. Morrison, Nature 501(2013) 328-337. doi: 10.1038/nature12624
4. [4]
  C. Ma, R. Fan, H. Ahmad, et al., Nat. Med. 17(2011) 738-743. doi: 10.1038/nm.2375
5. [5]
  K. Xu, G.S. Zhong, X.W. Zhuang, Science 339(2013) 452-456. doi: 10.1126/science.1232251
6. [6]
  V. Sanchez-Freire, A.D. Ebert, T. Kalisky, S.R. Quake, J.C. Wu, Nat. Protoc. 7(2012) 829-838. doi: 10.1038/nprot.2012.021
7. [7]
  V. Lecault, M. Vaninsberghe, S. Sekulovic, et al., Nat. Methods 8(2011) 581-586. doi: 10.1038/nmeth.1614
8. [8]
  X. Wei, D.H. Zheng, Y. Cai, et al., Anal. Chem. 90(2018) 14543-14550. doi: 10.1021/acs.analchem.8b04471
9. [9]
  A. Sarkar, S. Kolitz, D.A. Lauffenburger, J. Han, Nat. Commun. 5(2014) 3421. doi: 10.1038/ncomms4421
10. [10]
  P.C. Blainey, S.R. Quake, Nat. Methods 11(2014) 19-21. doi: 10.1038/nmeth.2783
11. [11]
  X. Wei, L.L. Hu, M.L. Chen, T. Yang, J.H. Wang, Anal. Chem. 88(2016) 12437-12444. doi: 10.1021/acs.analchem.6b03810
12. [12]
  L.A. Austin, B. Kang, M.A. El-Sayed, J. Am. Chem. Soc. 135(2013) 4688-4691. doi: 10.1021/ja4011145
13. [13]
  J.M. Camarillo, S. Swaminathan, N.A. Abshiru, et al., J. Am. Soc. Mass Spectrom. 30(2019) 2526-2534. doi: 10.1007/s13361-019-02255-x
14. [14]
  N.R. Munce, J. Li, P.R. Herman, L. Lilge, Anal. Chem. 76(2004) 4983-4989. doi: 10.1021/ac0496906
15. [15]
  W. Zhang, N. Li, L. Lin, H. Li, J.M. Lin, Anal. Chem. 93(2021) 6955-6960. doi: 10.1021/acs.analchem.0c05155
16. [16]
  V. Espina, J. Milia, G. Wu, S. Cowherd, L.A. Liotta, Methods Mol. Biol. 319(2006) 213-229. doi: 10.1007/978-1-59259-993-6_10
17. [17]
  X. Liu, Y. Jia, Z. Han, et al., Angew. Chem. Int. Ed. 60(2021) 12319-12322. doi: 10.1002/anie.202101293
18. [18]
  W.H. Tan, S. Takeuchi, Proc. Natl. Acad. Sci. U. S. A. 104(2007) 1146-1151. doi: 10.1073/pnas.0606625104
19. [19]
  S. Kobel, A. Valero, J. Latt, P. Renaud, M. Lutolf, Lab Chip 10(2010) 857-863. doi: 10.1039/b918055a
20. [20]
  K. Chung, C.A. Rivet, M.L. Kemp, H. Lu, Anal. Chem. 83(2011) 7044-7052. doi: 10.1021/ac2011153
21. [21]
  K. Chung, Y. Kim, J.S. Kanodia, et al., Nat Methods 8(2011) 171-176. doi: 10.1038/nmeth.1548
22. [22]
  K.K. Kabirov, I.M. Kapetanovic, A.V. Lyubimov, Chem. -Biol. Interact. 171(2008) 152-158. doi: 10.1016/j.cbi.2007.11.011
23. [23]
  Z. Ajtony, N. Szoboszlai, E.K. Suskó, et al., Talanta 76(2008) 627-634. doi: 10.1016/j.talanta.2008.04.014
1. [1]
  Dan Zhou , Liangjin Bao , Haoqi Long , Duo Zhou , Yuwei Xu , Bo Wang , Chuanqin Xia , Liang Xian , Chengbin Zheng . Capillary electrophoresis as sample introduction system for highly sensitive and interference-free determination of ⁹⁹Tc by ICP-MS. Chinese Chemical Letters, 2025, 36(4): 110093-. doi: 10.1016/j.cclet.2024.110093
2. [2]
  Min Gu , Huiwen Xiong , Liling Liu , Jilie Kong , Xueen Fang . Rapid Quantitative Detection of Procalcitonin by Microfluidics: An Instrumental Analytical Chemistry Experiment. University Chemistry, 2024, 39(4): 87-93. doi: 10.3866/PKU.DXHX202310120
3. [3]
  Kezuo Di , Jie Wei , Lijun Ding , Zhiying Shao , Junling Sha , Xilong Zhou , Huadong Heng , Xujing Feng , Kun Wang . A wearable sensor device based on screen-printed chip with biofuel cell-driven electrochromic display for noninvasive monitoring of glucose concentration. Chinese Chemical Letters, 2025, 36(2): 109911-. doi: 10.1016/j.cclet.2024.109911
4. [4]
  Pengyu Chen , Beibei Chen , Man He , Yuxi Zhou , Lei Lei , Jian Han , Bingsheng Zhou , Ligang Hu , Bin Hu . Nanoplastics and nano-ZnO facilitate Cd accumulation in zebrafish larvae via a distinct pathway: Revelation by LA-ICP-MS imaging. Chinese Chemical Letters, 2025, 36(2): 109908-. doi: 10.1016/j.cclet.2024.109908
5. [5]
  Yunan Yuan , Zhimin Luo , Jie Chen , Chaoliang He , Kai Hao , Huayu Tian . Constructing thermoresponsive PNIPAM-based microcarriers for cell culture and enzyme-free cell harvesting. Chinese Chemical Letters, 2024, 35(7): 109549-. doi: 10.1016/j.cclet.2024.109549
6. [6]
  Weiyu Chen , Zenghui Li , Chenguang Zhao , Lisha Zha , Junfeng Shi , Dan Yuan . Enzyme-modulate conformational changes in amphiphile peptide for selectively cell delivery. Chinese Chemical Letters, 2024, 35(12): 109628-. doi: 10.1016/j.cclet.2024.109628
7. [7]
  Zeyang Yao , Xinru You , Xudong Wang , Yunze Kang , Liying Wang , Ziji Zhang . Stem cell-based hydrogel for the repair and regeneration of cartilage. Chinese Chemical Letters, 2025, 36(8): 110607-. doi: 10.1016/j.cclet.2024.110607
8. [8]
  Zixuan Chen , Yafeng Wu , Zhaoyan Tian , Zhaohan Wang , Weiwei Liu , Songqin Liu . A reproducible hybrid membrane for in situ analysis of cell secretions with a wide size range. Chinese Chemical Letters, 2025, 36(12): 110917-. doi: 10.1016/j.cclet.2025.110917
9. [9]
  Yuanyi Zhou , Lili Wang , Li Chen , Qingbing Zha , Yu Meng , Mingshan Zhu . Functional inorganic nanomaterials for renal cell carcinoma treatment: Advancements and trends. Chinese Chemical Letters, 2025, 36(12): 110994-. doi: 10.1016/j.cclet.2025.110994
10. [10]
  Yukai Tong , Zhijun Wu , Bo Zhou , Min Hu , Anpei Ye . Surface tension of single suspended aerosol microdroplets. Chinese Chemical Letters, 2024, 35(4): 109062-. doi: 10.1016/j.cclet.2023.109062
11. [11]
  Kun Tang , Yu-Wu Zhong . Water reduction by an organic single-chromophore photocatalyst. Chinese Journal of Structural Chemistry, 2024, 43(8): 100376-100376. doi: 10.1016/j.cjsc.2024.100376
12. [12]
  Jianjun Liu , Xue Yang , Chi Zhang , Xueyu Zhao , Zhiwei Zhang , Yongmei Chen , Qinghong Xu , Shao Jin . Preparation and Fluorescence Characterization of CdTe Semiconductor Quantum Dots. University Chemistry, 2024, 39(7): 307-315. doi: 10.3866/PKU.DXHX202311031
13. [13]
  Weiwei He , Hongbo Zhang , Xudong Lin , Lili Zhu , Tingting Zheng , Hao Pei , Yang Tian , Min Zhang , Guoyue Shi , Lei Wu , Jianlong Zhao , Gulinuer Wumaier , Shengqing Li , Yufang Xu , Honglin Li , Xuhong Qian . Advancements in life-on-a-chip: The impact of "Beyond Limits Manufacturing" technology. Chinese Chemical Letters, 2024, 35(5): 109091-. doi: 10.1016/j.cclet.2023.109091
14. [14]
  Hongxia Li , Xiyang Wang , Du Qiao , Jiahao Li , Weiping Zhu , Honglin Li . Mechanism of nanoparticle aggregation in gas-liquid microfluidic mixing. Chinese Chemical Letters, 2024, 35(4): 108747-. doi: 10.1016/j.cclet.2023.108747
15. [15]
  Jun Lu , Jinrui Yan , Yaohao Guo , Junjie Qiu , Shuangliang Zhao , Bo Bao . Controlling solid form and crystal habit of triphenylmethanol by antisolvent crystallization in a microfluidic device. Chinese Chemical Letters, 2024, 35(4): 108876-. doi: 10.1016/j.cclet.2023.108876
16. [16]
  Gaowa Xing , Yuting Shang , Xiaorui Wang , Zengnan Wu , Qiang Zhang , Jiebing Ai , Qiaosheng Pu , Ling Lin . A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals. Chinese Chemical Letters, 2024, 35(10): 109491-. doi: 10.1016/j.cclet.2024.109491
17. [17]
  Cheng Wang , Ji Wang , Dong Liu , Zhi-Ling Zhang . Advances in virus-host interaction research based on microfluidic platforms. Chinese Chemical Letters, 2024, 35(12): 110302-. doi: 10.1016/j.cclet.2024.110302
18. [18]
  Dong Lv , Xuelei Liu , Wei Li , Qiang Zhang , Xinhong Yu , Yanchun Han . Single droplet formation by controlling the viscoelasticity of polymer solutions during inkjet printing. Chinese Chemical Letters, 2024, 35(6): 109401-. doi: 10.1016/j.cclet.2023.109401
19. [19]
  Zhihao Gu , Jiabo Le , Hehe Wei , Zehui Sun , Mahmoud Elsayed Hafez , Wei Ma . Unveiling the intrinsic properties of single NiZnFeO_x entity for promoting electrocatalytic oxygen evolution. Chinese Chemical Letters, 2024, 35(4): 108849-. doi: 10.1016/j.cclet.2023.108849
20. [20]
  Bei Li , Zhaoke Zheng . In situ monitoring of the spatial distribution of oxygen vacancies at the single-particle level. Chinese Journal of Structural Chemistry, 2024, 43(10): 100331-100331. doi: 10.1016/j.cjsc.2024.100331

Get Citation

PDF

XML

Metrics

PDF Downloads(3)
Abstract views(1521)
HTML views(133)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142