Citation: Huan Zhao, Enben Su, Li Huang, Yunfeng Zai, Yuan Liu, Zhu Chen, Song Li, Lian Jin, Yan Deng, Nongyue He. Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples[J]. Chinese Chemical Letters, ;2022, 33(2): 743-746. doi: 10.1016/j.cclet.2021.07.017 shu

Washing-free chemiluminescence immunoassay for rapid detection of cardiac troponin I in whole blood samples

Huan Zhao ^{a, b} ,
Enben Su ^{a, b, *} ,
Li Huang ^{a, b} ,
Yunfeng Zai ^{a, b} ,
Yuan Liu ^a ,
Zhu Chen ^c ,
Song Li ^c ,
Lian Jin ^{a, c, *} ,
Yan Deng ^{c, *} ,
Nongyue He ^{a, c, *}

a.
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Getein Biotechnology Co., Ltd., Nanjing 210000, China
c.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China

Received Date: 25 May 2021
Revised Date: 5 July 2021
Accepted Date: 7 July 2021
Available Online: 13 July 2021

Figures(4)

Chemiluminescence immunoassay (CLIA) has always been a great challenge in detecting cardiac troponin I (cTnI) in whole blood samples without centrifugation because of the interference of red blood cells and low sensitivity. In this study, the antigens and erythrocytes in the blood were captured by the antibodies immobilized on the magnetic particles, recognized by another biotin-conjugated cTnI antibody and detected by streptavidin/acridine aster-conjugated polychloromethylstyrene microspheres (PCMS). After magnetic separation, the supernatant was transferred and measured. No significant difference was noted between the cTnI concentrations of the serum samples, plasma samples and whole blood. The prepared PCMS provided more functional areas to conjugate streptavidin and acridinium ester, so the immunoassay has highly sensitive, the limits of blank at 0.012 ng/mL, and functional sensitivity at 0.019 ng/mL with a CV of 20%, and 0.058 ng/mL with a CV of 10%. Total precision of any sample type ranged from 2.62%~5.67%. The assay was linear over the studied range of 0.01-50.00 ng/mL, and no hook effect was found when cTnI concentrations reached 1900 ng/mL. No significant interference was noted with the potential endogenous interfering substances. Compared with the commercial kit (Abbott assay kit), the correlation coefficient was 0.9859. A washing-free CLIA was established for the rapid detection of cTnI in human whole blood, using erythrocyte capture antibodies-conjugated magnetic nanoparticles for eliminating the influence of erythrocytes and PCMS for signal amplification, which showed great potential in clinical application.
- Washing-free,
- Whole blood,
- Chemiluminescence immunoassay,
- Polychloromethylstyrene microspheres
1. [1]
  S.S. Virani, A. Alonso, E.J. Benjamin, et al., Circulation 141(2020) 139-151.
2. [2]
  A.S. Jaffe, V.C. Vasile, M. Milone, et al., J. Am. Coll. Cardiol. 58(2011) 1819-1824. doi: 10.1016/j.jacc.2011.08.026
3. [3]
  K. Thygesen, J.S. Alpert, A.S. Jaffe, et al., J. Am. Coll. Cardiol. 72(2018) 2231-2264. doi: 10.1016/j.jacc.2018.08.1038
4. [4]
  A. Wu, R. Christenson, D. Greene, et al., Clin. Chem. 64(2018) 645-655. doi: 10.1373/clinchem.2017.277186
5. [5]
  F.S. Apple, A.S. Jaffe, P. Collinson, et al., Clin. Biochem. 48(2015) 201-203. doi: 10.1016/j.clinbiochem.2014.08.021
6. [6]
  E.A. Amsterdam, N.K. Wenger, R.G. Brindis, et al., J. Am. Coll. Cardiol. 64(2014) 2645-2687. doi: 10.1016/j.jacc.2014.09.016
7. [7]
  K. Thygesen, J. Mair, E. Giannitsis, et al., Eur. Heart J. 33(2012) 2252-2257. doi: 10.1093/eurheartj/ehs154
8. [8]
  L. Jiao, L. Zhang, W. Du, et al., Nanoscale 11(2019) 8798-8802. doi: 10.1039/C8NR08741E
9. [9]
  H. Zhao, Q. Lin, L. Huang, et al., Nanoscale 13(2021) 3275-3284. doi: 10.1039/D0NR08008J
10. [10]
  L. Huang, Y. Zhang, E. Su, et al., Nanoscale Adv. 2(2020) 1138-1143. doi: 10.1039/C9NA00644C
11. [11]
  L. Huang, E. Su, Y. Liu, et al., Chin. Chem. Lett. 32(2021) 1555-1558. doi: 10.1016/j.cclet.2020.09.055
12. [12]
  H. Dong, C. Tang, Z. He, et al., Chin. Chem. Lett. 31(2020) 1812-1816. doi: 10.1016/j.cclet.2020.03.002
13. [13]
  C. Tang, Z. He, H. Liu, et al., J. Nanobiotechnol. 18(2020) 1-19. doi: 10.1186/s12951-019-0560-5
14. [14]
  M. Hussain, S. Zhu, P. Yang, et al., IEEE Access 8(2020) 107373-107386. doi: 10.1109/ACCESS.2020.3000357
15. [15]
  R. Bingisser, C. Cairns, M. Christ, et al., Am. J. Emerg. Med. 30(2012) 1639-1649. doi: 10.1016/j.ajem.2012.03.004
16. [16]
  K.D. Rooney, U.M. Schilling, Crit. Care 18(2014) 692. doi: 10.1186/s13054-014-0692-9
17. [17]
  A.T.F. Members, C.W. Hamm, J.P. Bassand, et al., Eur. Heart J. 32(2011) 2999-3054. doi: 10.1093/eurheartj/ehr236
18. [18]
  E.M. Antman, M. Cohen, P.J.L.M. Bernink, et al., JAMA 284(2000) 835-842. doi: 10.1001/jama.284.7.835
19. [19]
  S.R. Mehta, C.B. Granger, W.E. Boden, et al., N. Engl. J. Med. 360(2009) 2165-2175. doi: 10.1056/NEJMoa0807986
20. [20]
  S. Margel, E. Nov, I. Fisher, J. Polym. Sci. A: Polym. Chem. 29(1991) 347-355. doi: 10.1002/pola.1991.080290307
21. [21]
  M. Kedem, S. Margel, J. Polym. Sci. A: Polym. Chem. 40(2002) 1342-1352.
22. [22]
  L. Guo, H. Chen, N. He, Y. Deng, Chin. Chem. Lett. 29(2018) 1829-1833. doi: 10.1016/j.cclet.2018.10.038
23. [23]
  Z. Xiao, H. Chen, H. Chen, et al., J. Biomed. Nanotechnol. 15(2019) 1113-1134. doi: 10.1166/jbn.2019.2782
24. [24]
  Z. He, C. Tang, X. Chen, et al., Mater. Express 9(2019) 956-961. doi: 10.1166/mex.2019.1579
25. [25]
  Y. Fang, H. Liu, Y. Wang, et al., J. Biomed. Nanotechnol. 17(2021) 407-415. doi: 10.1166/jbn.2021.3028
26. [26]
  X. Mou, D. Sheng, Z. Chen, et al., J. Biomed. Nanotechnol. 15(2019) 2393-2400. doi: 10.1166/jbn.2019.2862
27. [27]
  X. Mou, Z. Chen, T. Li, et al., J. Biomed. Nanotechnol. 15(2019) 1832-1838. doi: 10.1166/jbn.2019.2802
28. [28]
  Z. Xiao, G. Yang, D. Yan, et al., Mater. Express 9(2019) 509-516. doi: 10.1166/mex.2019.1520
1. [1]
  Ruixin Liu , Feng Shi , Yanping Xia , Haibing Zhu , Jiawen Cao , Kai Peng , Chuanli Ren , Juan Li , Zhanjun Yang . Universal MOF nanozyme-induced catalytic amplification strategy for label-free electrochemical immunoassay. Chinese Chemical Letters, 2024, 35(11): 109664-. doi: 10.1016/j.cclet.2024.109664
2. [2]
  Han Wu , Yumei Wang , Zekai Ren , Hailin Cong , Youqing Shen , Bing Yu . The nanocarrier strategy for crossing the blood-brain barrier in glioma therapy. Chinese Chemical Letters, 2025, 36(4): 109996-. doi: 10.1016/j.cclet.2024.109996
3. [3]
  Gaowa Xing , Yuting Shang , Xiaorui Wang , Zengnan Wu , Qiang Zhang , Jiebing Ai , Qiaosheng Pu , Ling Lin . A microfluidic biosensor for multiplex immunoassay of foodborne pathogens agitated by programmed audio signals. Chinese Chemical Letters, 2024, 35(10): 109491-. doi: 10.1016/j.cclet.2024.109491
4. [4]
  Keqiang Shi , Xiujuan Hong , Dongyan Xu , Tao Pan , Huiwen Wang , Hongru Feng , Cheng Guo , Yuanjiang Pan . Analysis of RNA modifications in peripheral white blood cells from breast cancer patients by mass spectrometry. Chinese Chemical Letters, 2025, 36(3): 110079-. doi: 10.1016/j.cclet.2024.110079
5. [5]
  Shaobin He , Xiaoyun Guo , Qionghua Zheng , Huanran Shen , Yuan Xu , Fenglin Lin , Jincheng Chen , Haohua Deng , Yiming Zeng , Wei Chen . Engineering nickel-supported osmium bimetallic nanozymes with specifically improved peroxidase-like activity for immunoassay. Chinese Chemical Letters, 2025, 36(4): 110096-. doi: 10.1016/j.cclet.2024.110096
6. [6]
  Xueyan Zhang , Jicong Chen , Songren Han , Shiyan Dong , Huan Zhang , Yuhong Man , Jie Yang , Ye Bi , Lesheng Teng . The size-switchable microspheres co-loaded with RANK siRNA and salmon calcitonin for osteoporosis therapy. Chinese Chemical Letters, 2024, 35(12): 109668-. doi: 10.1016/j.cclet.2024.109668
7. [7]
  Zhefei Hu , Jingwen Liao , Jiawen Zhou , Lulu Zhao , Yanjuan Liu , Yuefei Zhang , Wei Chen , Sheng Tang . A new green approach to synthesizing MIP-202@porous silica microspheres for positional isomer/enantiomer/hydrophilic separation. Chinese Chemical Letters, 2025, 36(1): 109985-. doi: 10.1016/j.cclet.2024.109985
8. [8]
  Rui Li , Ruijie Lu , Libin Yang , Jianwen Li , Zige Guo , Qiquan Yan , Mengjun Li , Yazhuo Ni , Keying Chen , Yaoyang Li , Bo Xu , Mengzhen Cui , Zhan Li , Zhiying Zhao . Immobilization of chitosan nano-hydroxyapatite alendronate composite microspheres on polyetheretherketone surface to enhance osseointegration by inhibiting osteoclastogenesis and promoting osteogenesis. Chinese Chemical Letters, 2025, 36(4): 110242-. doi: 10.1016/j.cclet.2024.110242
9. [9]
  Jisheng Liu , Junli Chen , Xifeng Zhang , Yin Wu , Xin Qi , Jie Wang , Xiang Gao . Red blood cell membrane-coated FLT3 inhibitor nanoparticles to enhance FLT3-ITD acute myeloid leukemia treatment. Chinese Chemical Letters, 2024, 35(9): 109779-. doi: 10.1016/j.cclet.2024.109779
10. [10]
  Lei Shen , Hongmei Liu , Ming Jin , Jinchao Zhang , Caixia Yin , Shuxiang Wang , Yutao Yang . “Three-in-one” strategy of trifluoromethyl regulated blood-brain barrier permeable fluorescent probe for peroxynitrite and antiepileptic evaluation of edaravone. Chinese Chemical Letters, 2024, 35(10): 109572-. doi: 10.1016/j.cclet.2024.109572
11. [11]
  Lishan Xiong , Xinyuan Li , Xiaojie Lu , Zhendong Zhang , Yan Zhang , Wen Wu , Chenhui Wang . Inhaled multilevel size-tunable, charge-reversible and mucus-traversing composite microspheres as trojan horse: Enhancing lung deposition and tumor penetration. Chinese Chemical Letters, 2024, 35(9): 109384-. doi: 10.1016/j.cclet.2023.109384
12. [12]
  Gengchen Guo , Tianyu Zhao , Ruichang Sun , Mingzhe Song , Hongyu Liu , Sen Wang , Jingwen Li , Jingbin Zeng . Au-Fe₃O₄ dumbbell-like nanoparticles based lateral flow immunoassay for colorimetric and photothermal dual-mode detection of SARS-CoV-2 spike protein. Chinese Chemical Letters, 2024, 35(6): 109198-. doi: 10.1016/j.cclet.2023.109198
13. [13]
  Donghui Wu , Qilin Zhao , Jian Sun , Xiurong Yang . Corrigendum to 'Fluorescence immunoassay based on alkaline phosphatase-induced in situ generation of fluorescent non-conjugated polymer dots' [Chin. Chem. Lett. 34 (2023) 107672]. Chinese Chemical Letters, 2024, 35(12): 109881-. doi: 10.1016/j.cclet.2024.109881
14. [14]
  Zhuangzhuang Zhang , Yaru Qiao , Jun Zhao , Dai-Huo Liu , Mengmin Jia , Hongwei Tang , Liang Wang , Dongmei Dai , Bao Li . Fluorine-doped K_0.39Mn_0.77Ni_0.23O_1.9F_0.1 microspheres with highly reversible oxygen redox reaction for potassium-ion battery cathode. Chinese Chemical Letters, 2025, 36(3): 109907-. doi: 10.1016/j.cclet.2024.109907
15. [15]
  Ruiheng Liang , Huizhong Wu , Zhongzheng Hu , Ge Song , Xuyang Zhang , Omotayo A. Arotiba , Minghua Zhou . Hierarchical Fe-Bi/Bi₇O₉I₃/OVs microspheres coupled with natural air diffusion electrode to achieve efficient heterogeneous visible-light-driven photoelectro-Fenton degradation of tetracycline without aeration. Chinese Chemical Letters, 2025, 36(4): 110136-. doi: 10.1016/j.cclet.2024.110136
16. [16]
  Qiangwei Wang , Huijiao Liu , Mengjie Wang , Haojie Zhang , Jianda Xie , Xuanwei Hu , Shiming Zhou , Weitai Wu . Observation of high ionic conductivity of polyelectrolyte microgels in salt-free solutions. Chinese Chemical Letters, 2024, 35(4): 108743-. doi: 10.1016/j.cclet.2023.108743
17. [17]
  Gongcheng Ma , Qihang Ding , Yuding Zhang , Yue Wang , Jingjing Xiang , Mingle Li , Qi Zhao , Saipeng Huang , Ping Gong , Jong Seung Kim . Palladium-free chemoselective probe for in vivo fluorescence imaging of carbon monoxide. Chinese Chemical Letters, 2024, 35(9): 109293-. doi: 10.1016/j.cclet.2023.109293
18. [18]
  Haiying Lu , Weijie Li . The electrolyte solvation and interfacial chemistry for anode-free sodium metal batteries. Chinese Journal of Structural Chemistry, 2024, 43(11): 100334-100334. doi: 10.1016/j.cjsc.2024.100334
19. [19]
  Tao Wei , Jiahao Lu , Pan Zhang , Qi Zhang , Guang Yang , Ruizhi Yang , Daifen Chen , Qian Wang , Yongfu Tang . An intermittent lithium deposition model based on bimetallic MOFs derivatives for dendrite-free lithium anode with ultrahigh areal capacity. Chinese Chemical Letters, 2024, 35(8): 109122-. doi: 10.1016/j.cclet.2023.109122
20. [20]
  Chunxiu Yu , Zelin Wu , Hongle Shi , Lingyun Gu , Kexin Chen , Chuan-Shu He , Yang Liu , Heng Zhang , Peng Zhou , Zhaokun Xiong , Bo Lai . Insights into the electron transfer mechanisms of peroxydisulfate activation by modified metal-free acetylene black for degradation of sulfisoxazole. Chinese Chemical Letters, 2024, 35(8): 109334-. doi: 10.1016/j.cclet.2023.109334

Get Citation

PDF

XML

Metrics

PDF Downloads(7)
Abstract views(883)
HTML views(65)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142