Citation: Guangzhong Yin, Jingjing Wei, Yu Shao, Wen-Hao Wu, Lianjie Xu, Wen-Bin Zhang. Native conjugation between proteins and [60]fullerene derivatives using SpyTag as a reactive handle[J]. Chinese Chemical Letters, ;2021, 32(1): 353-356. doi: 10.1016/j.cclet.2020.04.034 shu

Native conjugation between proteins and [60]fullerene derivatives using SpyTag as a reactive handle

Guangzhong Yin ^{a, b, 1} ,
Jingjing Wei ^{a, b, 1} ,
Yu Shao ^a ,
Wen-Hao Wu ^a ,
Lianjie Xu ^a ,
Wen-Bin Zhang ^{a, *,}

a.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of the Ministry of Education, College of Chemistry and Molecular Engineering, Center for Soft Matter Science and Engineering, Peking University, Beijing 100871, China
b.
College of Chemical and Environmental Engineering, Anyang Institute of Technology, Anyang 455000, China

wenbin@pku.edu.cn

Received Date: 12 March 2020
Revised Date: 9 April 2020
Accepted Date: 21 April 2020
Available Online: 26 April 2020

Figures(3)

Herein, we report the facile conjugation between proteins and water-soluble [60]fullerene derivatives (DC₆₀) under native conditions using SpyTag as a reactive handle. Water-soluble [60]fullerene derivatives were first prepared via sequential Bingel-Hirsch reaction and pclickedq with SpyTag to give DC₆₀-SpyTag for native conjugation with proteins by the highly efficient SpyTag-SpyCatcher chemistry. The bioconjugation was confirmed by MALDI-TOF MS spectra and SDS-PAGE analysis. The TEM and UV-vis spectroscopic study further revealed that the DC₆₀ could alter the optical performance and induce aggregation of the target proteins. It thus provides a general and robust method for modifying proteins with C₆₀ derivatives and could potentially be adapted for native conjugation between proteins and other nonbiological motifs as well.
- SpyTag,
- SpyCatcher,
- Fullerene,
- Protein,
- Bioconjugate
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