Citation: Liu Yuan, Li Taotao, Ling Chuxuan, Wang Zunliang, Jin Lian, Zhao Yongxiang, Chen Zhu, Li Song, Deng Yan, He Nongyue. A simple visual method for DNA detection based on the formation of gold nanoparticles[J]. Chinese Chemical Letters, ;2019, 30(12): 2359-2362. doi: 10.1016/j.cclet.2019.10.033 shu

A simple visual method for DNA detection based on the formation of gold nanoparticles

Liu Yuan ^a,1 ,
Li Taotao ^b,1 ,
Ling Chuxuan ^c ,
Wang Zunliang ^a ,
Jin Lian ^d ,
Zhao Yongxiang ^e ,
Chen Zhu ^a,d ,
Li Song ^d ,
Deng Yan ^a,d,*, ,
He Nongyue ^a,e,*,

a.
State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
b.
Hunan Provincial Key Lab of Dark Tea and Jin-Hua, School of Materials and Chemical Engineering, Hunan City University, Yiyang 413000, China
c.
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
d.
Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, China
e.
National Center for International Bio-targeting Theranostics, Guangxi Key Laboratory of Bio-targeting Theranostics, Collaborative Innovation Center for Targeting Tumor Theranostics, Guangxi Medical University, Nanning 530021, China

hndengyan@126.com

nyhe1958@163.com

Received Date: 26 September 2019
Revised Date: 25 October 2019
Accepted Date: 25 October 2019
Available Online: 2 December 2019

Figures(7)

A simple visual method for DNA detection during the formation of gold nanoparticles (AuNPs) was developed based on different electrostatic properties of single strand DNA (ssDNA) and double strand DNA (dsDNA). Since the ssDNA is easy to bind to AuNPs due to its exposed bases which could prevent saltinduced aggregation of AuNPs. The dsDNA always present negative charge because its negatively charged phosphate backbone is exposed. In this case, the dsDNA could disturb the adsorption between dsDNA and AuNPs and result in non-aggregation of AuNPs. After hybridization, chloroauric acid and ascorbic acid were added to the mixture solution, and the solution changed to red immediately and turned to purple in 10 min in the present of target DNA. TEM results confirmed that the change of color stemed from aggregation of AuNPs. In order to obtain accurate results by naked eye, the DNA detection assay should be conducted under pH 7.0.
- Visual method,
- Unmodified AuNPs,
- DNA,
- Hybridization adsorption,
- Detection
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