Citation: Jiang Chuankun, Li Longchun, Jiang Jiacheng, Hou Lilin, Fang Gemin, Yu Haizhu, Meng Xiangming. A bright two-photon fluorescent probe for real-time monitoring autophagy in living cells[J]. Chinese Chemical Letters, ;2020, 31(2): 447-450. doi: 10.1016/j.cclet.2019.07.046 shu

A bright two-photon fluorescent probe for real-time monitoring autophagy in living cells

Jiang Chuankun ^a,1 ,
Li Longchun ^b,1, ,
Jiang Jiacheng ^a ,
Hou Lilin ^a ,
Fang Gemin ^a,c ,
Yu Haizhu ^a,*, ,
Meng Xiangming ^a,b,*,

a.
School of Chemistry and Chemical Engineering, Anhui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, and Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China
b.
Department of Applied Chemistry, School of science, Anhui Agricultural University, Hefei 230036, China
c.
Institute of Physical Science and Information Technology, Anhui University, Hefei 230601, China

llch75@163.com

yuhaizhu@ahu.edu.cn

mengxm@ahu.edu.cn

Received Date: 4 June 2019
Revised Date: 22 July 2019
Accepted Date: 23 July 2019
Available Online: 25 July 2019

Figures(6)

A novel donor-acceptor (D-A) type of two-photon (TP) fluorescent probe, i.e. Lyso-OSC, based on the lysosome-targeting morpholine group was developed. The polarity sensing coumarin group was functionalized as the acceptor and the 1-vinyl-4-methoxybenzene group was engineered as the donor. The fluorescence intensity and emission maximum wavelength of Lyso-OSC are highly sensitive to the polarity changes of solvent. The two-photon absorption cross-section and tissue penetration depth are up to 254 GM and 150 μm, respectively. The strong fluorescence, high sensitivity to polarity, low cytotoxicity, and accurate lysosome-targeting ability entail Lyso-OSC the excellent performance in detecting the polarity changes of cellular environment. To this end, a bright, real-time imaging autophagy of living cells has been achieved.
- Two-photon,
- Lysosome-targeting,
- Polarity,
- Real-time,
- Autophagy
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