Citation: Qi He, Li Zheng, Zheng Haijiao, Fu Li, Jia Qiong. Facile preparation of hydrophilic glutathione modified magnetic nanomaterials for specific enrichment of glycopeptides[J]. Chinese Chemical Letters, ;2019, 30(12): 2181-2185. doi: 10.1016/j.cclet.2019.06.046 shu

Facile preparation of hydrophilic glutathione modified magnetic nanomaterials for specific enrichment of glycopeptides

Qi He ^a ,
Li Zheng ^a ,
Zheng Haijiao ^a ,
Fu Li ^b,**, ,
Jia Qiong ^a,c,*,

a.
College of Chemistry, Jilin University, Changchun 130012, China
b.
The Second Hospital of Jilin University, Changchun 130041, China
c.
Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, School of Life Sciences, Jilin University, Changchun 130012, China

^**

doctorfuli@sina.com

jiaqiong@jlu.edu.cn

Received Date: 1 May 2019
Revised Date: 21 June 2019
Accepted Date: 24 June 2019
Available Online: 25 December 2019

Figures(4)

Investigations of glycosylated proteins or peptides and their related biological pathways provide new possibilities for illuminating the physiological and pathological mechanisms of glycosylation modification. However, open-ended and in-depth analysis of glycoproteomics is usually subjected to the low-abundance of glycopeptides, heterogeneous glycans, and a variety of interference molecules. In order to alleviate the influence of these obstacles, effective preconcentration of glycopeptides are indispensable. Here, we employed a hydrophilic interaction liquid chromatography (HILIC)-based method to universally capture glycopeptides. Glutathione modified magnetic nanoparticles (Fe₃O₄@AuGSH) were synthesized through a simple process and exploited to enrich glycopeptides from complex samples. The prepared materials showed excellent ability to trap glycopeptides from standard glycoproteins digests, low detection limit (10 fmol/μL), and good selectivity (HRP:BSA=1:100). These results indicated that glutathione-based magnetic nanoparticles synthesized in this work had great potential for glycopeptides enrichment.
- Hydrophilic,
- Glutathione,
- Magnetic,
- Enrichment,
- Glycopeptides
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沈阳化工大学材料科学与工程学院沈阳 110142