Citation: Cao Yongjin, Zheng Min, Cai Weihua, Wang Zongcai. Enzyme-loaded liposome with biocatalytic precipitation for potentiometric immunoassay of thyroid-stimulating hormone in thyroid carcinoma[J]. Chinese Chemical Letters, ;2020, 31(2): 463-467. doi: 10.1016/j.cclet.2019.06.024 shu

Enzyme-loaded liposome with biocatalytic precipitation for potentiometric immunoassay of thyroid-stimulating hormone in thyroid carcinoma

a.
Department of Thyroid and Breast Surgery, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China
b.
Department of Medical Oncology, The First Affiliated Hospital of Fujian Medical University, Fuzhou 350005, China

zhengmin1981@163.com

Received Date: 8 May 2019
Revised Date: 11 June 2019
Accepted Date: 13 June 2019
Available Online: 14 June 2016

Figures(4)

A simple and feasible potentiometric immunosensing platform based on enzymatic biocatalytic precipitation technique was designed for the sensitive detection of thyroid-stimulating hormone (TSH; a typical kind of biomarkers for thyroid carcinoma), using horseradish peroxidase (HRP)-loaded liposome for the signal amplification. To construct such an assay system, a sandwich-type immunoreaction was readily carried out on monoclonal anti-TSH capture antibody-coated electrode by using polyclonal antiTSH secondary antibody-conjugated HRP-loaded liposome. Accompanying the formation of sandwichtype immunocomplex, the carried liposome was lysed through the added Triton X-100 to release the entrapped HRP molecules, which catalyzed the oxidation of 4-chloro-1-naphthol to produce an insoluble and uncharged organic precipitation on the electrode surface, thereby causing the change of the local electrical potential. Two labeling protocols with and without the liposome were investigated for detection of target TSH, improved analytical features were achieved with HRP-entrapped liposome. Under optimal conditions, the potentiometric immunosensor had good responses for TSH detection within the linear range of 0.01-30 μIU/mL at a detection limit of 0.0067 μIU/mL. Good reproducibility, high specificity and long-time stability were acquired during the assay procedure. Importantly, a wellmatched accuracy between the potentiometric immunosensor and commercial human TSH ELISA kit was gave for the analysis of human serum samples.
- Potentiometer,
- Modification electrode,
- Potentiometric immunoassay,
- Thyroid-stimulating hormone,
- Enzyme-encapsulated liposome,
- Enzyme biocatalytic precipitation
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沈阳化工大学材料科学与工程学院沈阳 110142