Citation: Zhou Peng, Lv Pei, Yu Lu, Liu Sanling, Zhang Longhua, Tian Changlin. Fluorescence lifetime based distance measurement illustrates conformation changes of PYL10-CL2 upon ABA binding in solution state[J]. Chinese Chemical Letters, ;2019, 30(5): 1067-1070. doi: 10.1016/j.cclet.2019.01.020 shu

Fluorescence lifetime based distance measurement illustrates conformation changes of PYL10-CL2 upon ABA binding in solution state

High Magnetic Field Laboratory, Chinese Academy of Sciences and School of Life Sciences, University of Science and Technology of China, Hefei 230027, China

zlhustc@ustc.edu.cn

cltian@ustc.edu.cn

Received Date: 22 December 2018
Revised Date: 18 January 2019
Accepted Date: 22 January 2019
Available Online: 22 May 2019

Figures(3)

Förster resonance energy transfer (FRET) is a widely used distance measurement method to illustrate protein conformational dynamics. The FRET method relies on the distance between donor and acceptor, as well as the labelling efficiency, the size and the properties of the fluorophores. Here, we labelled a pair of small fluorophores and calculated the energy transferred efficiency through fluorescence lifetime analysis, which can provide more reliable distance measurement than intensity attenuation. The donor fluorophore, 7-hydroxycoumarin-4-yl-ethylglycine (HC), was genetically incorporated into specific sites of PYL10, obtaining complete labelling efficiency. The acceptor fluorophore, Alexa488, was labelled through the disulfide bond, whose labelling efficiency was estimated through both absorption peaks and lifetime populations. Fluorescence lifetime and anisotropy analysis showed ABA-induced local conformation changes and dynamics of several HC incorporation sites of PYL10. The lifetime-based FRET distance measurement illustrated the conformation changes of PYL10 with or without ABA application, which is consistent with the previously reported crystal structures.
- Fluorescence lifetime,
- Anisotropy,
- Unnatural amino acid,
- PYL10,
- Distance measurement
1. [1]
  R.M. Clegg, Curr. Opin. Biotechnol. 6(1995) 103-110. doi: 10.1016/0958-1669(95)80016-6
2. [2]
  V.E. Isaac, L. Patel, T. Curran, C. Abate-Shen, Biochemistry 34(1995) 15276-15281. doi: 10.1021/bi00046a036
3. [3]
  L. Ma, F. Yang, J. Zheng, J. Mol. Struct. 1077(2014) 87-100. doi: 10.1016/j.molstruc.2013.12.071
4. [4]
  E.A. Jares-Erijman, T.M. Jovin, Nat. Biotechnol. 21(2003) 1387-1395. doi: 10.1038/nbt896
5. [5]
  A. Margineanu, J.J. Chan, D.J. Kelly, et al., Sci. Rep. 6(2016) 28186. doi: 10.1038/srep28186
6. [6]
  H.S. Chung, J.M. Louis, I.V. Gopich, J. Phys. Chem. B 120(2016) 680-699. doi: 10.1021/acs.jpcb.5b11351
7. [7]
  H.J. Carlson, R.E. Campbell, Curr. Opin. Biotechnol. 20(2009) 19-27. doi: 10.1016/j.copbio.2009.01.003
8. [8]
  S.J. Peng, R.R. Sun, W.J. Wang, C.L. Chen, Chin. Chem. Lett. 29(2018) 1503-1508. doi: 10.1016/j.cclet.2017.12.006
9. [9]
  C. Albrecht, R. Joseph, Lakowicz, Anal. Bioanal. Chem. 390(2008) 1223-1224. doi: 10.1007/s00216-007-1822-x
10. [10]
  M.G. Zhang, M. Wen, Y. Xiong, L.H. Zhang, C.L. Tian, Chin. Chem. Lett. 29(2018) 1509-1512. doi: 10.1016/j.cclet.2018.04.026
11. [11]
  T. Koopmans, M. van Haren, L.Q. van Ufford, J.M. Beekman, N.I. Martin, Bioorg. Med. Chem. 21(2013) 553-559. doi: 10.1016/j.bmc.2012.10.055
12. [12]
  M. Braun, T. Dittrich, Beilstein J. Org. Chem. 6(2010) 69.
13. [13]
  W.J. Qin, C.C. Xu, Y.F. Zhao, et al., Chin. Chem. Lett. 29(2018) 1451-1455. doi: 10.1016/j.cclet.2018.04.007
14. [14]
  T.S. Young, I. Ahmad, J.A. Yin, P.G. Schultz, J. Mol. Biol. 395(2010) 361-374. doi: 10.1016/j.jmb.2009.10.030
15. [15]
  S.M. Wang, Y.A. Zhang, L.H. Zhang, M. Zhang, C.L. Tian, Chin. Chem. Lett. 29(2018) 1513-1516. doi: 10.1016/j.cclet.2018.08.002
16. [16]
  S.L. Liu, P. Lv, D. Li, et al., Chem. Commun. (Camb.) 51(2015) 15971-15974. doi: 10.1039/C5CC06124E
17. [17]
  D. Li, Y.A. Zhang, Y. He, et al., Protein Cell 9(2018) 592. doi: 10.1007/s13238-017-0389-3
18. [18]
  W.H. Cheng, A. Endo, L. Zhou, et al., Plant Cell 14(2002) 2723-2743. doi: 10.1105/tpc.006494
19. [19]
  A. Christmann, D. Moes, A. Himmelbach, et al., Plant Biol. (Stuttg.) 8(2006) 314-325. doi: 10.1055/s-2006-924120
20. [20]
  Y. Ma, I. Szostkiewicz, A. Korte, et al., Science 324(2009) 1064-1068.
21. [21]
  S.Y. Park, P. Fung, N. Nishimura, et al., Science 324(2009) 1068-1071.
22. [22]
  D. Sun, H. Wang, M. Wu, et al., Biochem. Biophys. Res. Commun. 418(2012) 122-127. doi: 10.1016/j.bbrc.2011.12.145
23. [23]
  Q. Hao, P. Yin, W. Li, et al., Mol. Cell 42(2011) 662-672. doi: 10.1016/j.molcel.2011.05.011
24. [24]
  M. Ameloot, M. vandeVen, A.U. Acuna, B. Valeur, Pure Appl. Chem. 85(2013) 589-608. doi: 10.1351/PAC-REP-11-11-12
1. [1]
  Jia-Li Xie , Tian-Jin Xie , Yu-Jie Luo , Kai Mao , Cheng-Zhi Huang , Yuan-Fang Li , Shu-Jun Zhen . Octopus-like DNA nanostructure coupled with graphene oxide enhanced fluorescence anisotropy for hepatitis B virus DNA detection. Chinese Chemical Letters, 2024, 35(6): 109137-. doi: 10.1016/j.cclet.2023.109137
2. [2]
  Luolin Wang , Xing Liang , Hanwen Chi , Weiying Lin . A dual-targeted near-infrared fluorescence lifetime probe for detecting viscosity heterogeneity in arthritic mice. Chinese Chemical Letters, 2025, 36(12): 110962-. doi: 10.1016/j.cclet.2025.110962
3. [3]
  Ke Xu , Shulai Lei , Panshuo Wang , Weiyi Wang , Yuan Feng , Junsheng Feng . Unraveling the microscopic origin of out of plane magnetic anisotropy in Ⅵ₃. Chinese Chemical Letters, 2025, 36(8): 110257-. doi: 10.1016/j.cclet.2024.110257
4. [4]
  Jiajia Zhuang , Chunyu Cui , Changjiang Li , Gang Luo , Jiaping Tong , Di Sun . Counter-ion effect to the Ising-type magnetic anisotropy and magnetic relaxation in trigonal bipyramidal Co(Ⅱ) complexes. Chinese Chemical Letters, 2025, 36(7): 110091-. doi: 10.1016/j.cclet.2024.110091
5. [5]
  Nanjiong Pang , Yunxiang He , Mingyao Wang , Xiaoling Wang , Junling Guo , Xuepin Liao , Bi Shi . Exploiting selective isotope exchange of amino–phenolic networks for boron-10 isotopologue separation. Chinese Chemical Letters, 2025, 36(10): 110914-. doi: 10.1016/j.cclet.2025.110914
6. [6]
  Yuxin Xiao , Xiaowei Wang , Yutong Yin , Fangchao Yin , Jinchao Li , Zhiyuan Hou , Mashooq Khan , Rusong Zhao , Wenli Wu , Qiongzheng Hu . Distance-based lateral flow biosensor for the quantitative detection of bacterial endotoxin. Chinese Chemical Letters, 2024, 35(12): 109718-. doi: 10.1016/j.cclet.2024.109718
7. [7]
  Shaofeng Gong , Zi-Wei Deng , Chao Wu , Wei-Min He . Stabilized carbon radical-mediated three-component functionalization of amino acid/peptide derivatives. Chinese Chemical Letters, 2025, 36(5): 110936-. doi: 10.1016/j.cclet.2025.110936
8. [8]
  Qiang Luo , Jinfeng Sun , Zhibo Li , Bin Liu , Jianxun Ding . Thermo-sensitive poly(amino acid) hydrogel mediates cytoprotection through an antioxidant mechanism. Chinese Chemical Letters, 2025, 36(7): 110433-. doi: 10.1016/j.cclet.2024.110433
9. [9]
  Qi Wei , Hua Xin , Xiaolong Wang , Changjuan Qin , Yuanzhen Su , Di Li , Jianxun Ding . Unimolecular chiral poly(amino acid)s as adjuvants of nanovaccines for augmented cancer immunotherapy. Chinese Chemical Letters, 2025, 36(11): 111477-. doi: 10.1016/j.cclet.2025.111477
10. [10]
  Kaimin WANG , Xiong GU , Na DENG , Hongmei YU , Yanqin YE , Yulu MA . Synthesis, structure, fluorescence properties, and Hirshfeld surface analysis of three Zn(Ⅱ)/Cu(Ⅱ) complexes based on 5-(dimethylamino) isophthalic acid. Chinese Journal of Inorganic Chemistry, 2024, 40(7): 1397-1408. doi: 10.11862/CJIC.20240009
11. [11]
  Yiyue Ding , Qiuxiang Zhang , Lei Zhang , Qilu Yao , Gang Feng , Zhang-Hui Lu . Exceptional activity of amino-modified rGO-immobilized PdAu nanoclusters for visible light-promoted dehydrogenation of formic acid. Chinese Chemical Letters, 2024, 35(7): 109593-. doi: 10.1016/j.cclet.2024.109593
12. [12]
  Yong-Fang Shi , Sheng-Hua Zhou , Zuju Ma , Xin-Tao Wu , Hua Lin , Qi-Long Zhu . From [Ba3S][GeS4] to [Ba3CO3][MS4] (M = Ge, Sn): Enhancing optical anisotropy in IR birefringent crystals via functional group implantation. Chinese Journal of Structural Chemistry, 2025, 44(1): 100455-100455. doi: 10.1016/j.cjsc.2024.100455
13. [13]
  Zeyin Chen , Jiaju Shi , Yusheng Zhou , Peng Zhang , Guodong Liang . Polymer microparticles with ultralong room-temperature phosphorescence for visual and quantitative detection of oxygen through phosphorescence image and lifetime analysis. Chinese Chemical Letters, 2025, 36(5): 110629-. doi: 10.1016/j.cclet.2024.110629
14. [14]
  Yanjie Li , Chaoqun Qu , Siqi Meng , Jiaqi Hu , Ze Gao , Hongji Xu , Rui Gao , Ming Feng . Revealing electronic state evolution of Co(Ⅱ)/Co(Ⅲ) in CoO (111) plane during OER process through magnetic measurement. Chinese Chemical Letters, 2025, 36(3): 109872-. doi: 10.1016/j.cclet.2024.109872
15. [15]
  Wenqing Deng , Fanfeng Deng , Ting Zhang , Junjie Lin , Liang Zhao , Gang Li , Yi Pan , Jiebin Yang . Continuous measurement of reactive ammonia in hydrogen fuel by online dilution module coupled with Fourier transform infrared spectrometer. Chinese Chemical Letters, 2025, 36(3): 110085-. doi: 10.1016/j.cclet.2024.110085
16. [16]
  Xuan Song , Teng Fu , Yajie Yang , Yahan Kuang , Xiuli Wang , Yu-Zhong Wang . Spatial-confinement combustion strategy enabling free radicals chemiluminescence direct-measurement in flame-retardant mechanism. Chinese Chemical Letters, 2025, 36(5): 110699-. doi: 10.1016/j.cclet.2024.110699
17. [17]
  Shuangying Li , Qingxiang Zhou , Zhi Li , Menghua Liu , Yanhui Li . Sensitive measurement of silver ions in environmental water samples integrating magnetic ion-imprinted solid phase extraction and carbon dot fluorescent sensor. Chinese Chemical Letters, 2024, 35(5): 108693-. doi: 10.1016/j.cclet.2023.108693
18. [18]
  Kang Wei , Jiayu Li , Wen Zhang , Bing Yuan , Ming-De Li , Pingwu Du . A strained π-extended [10]cycloparaphenylene carbon nanoring. Chinese Chemical Letters, 2024, 35(5): 109055-. doi: 10.1016/j.cclet.2023.109055
19. [19]
  Juan Guo , Mingyuan Fang , Qingsong Liu , Xiao Ren , Yongqiang Qiao , Mingju Chao , Erjun Liang , Qilong Gao . Zero thermal expansion in Cs₂W₃O₁₀. Chinese Chemical Letters, 2024, 35(7): 108957-. doi: 10.1016/j.cclet.2023.108957
20. [20]
  . . University Chemistry, 2024, 39(10): 0-0.

Get Citation

PDF

XML

Metrics

PDF Downloads(2)
Abstract views(1351)
HTML views(12)

通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142