Citation: Zhao Lifang, Teng Long, Zhang Juan, Li He. Point-of-care detection of Microcystin-LR with a personal glucose meter in drinking water source[J]. Chinese Chemical Letters, ;2019, 30(5): 1035-1037. doi: 10.1016/j.cclet.2019.01.001 shu

Point-of-care detection of Microcystin-LR with a personal glucose meter in drinking water source

Zhao Lifang ^a,b ,
Teng Long ^c ,
Zhang Juan ^b ,
Li He ^a,*,

a.
School of Chemistry and Chemical Engineering, University of Jinan, Ji'nan 250022, China
b.
Shandong Product Quality Inspection Research Institute, Ji'nan 250102, China
c.
Environmental Engineering Co., Ltd. of Shandong Academy of Environmental Sciences, Ji'nan 250103, China

lihecd@gmail.com

Received Date: 10 December 2018
Revised Date: 1 January 2019
Accepted Date: 4 January 2019
Available Online: 4 May 2019

Figures(3)

In this study, a point-of-care sensing protocol has been reported for rapid and sensitive detection of Microcystin-LR (MC-LR) in water by personal glucose meter. The proposed immunosensor has been fabricated by using a primary antibody coated ZnFe₂O₄ nanoparticles to capture the target MC-LR. Consequently, the invertase@secondary antibody-conjugated graphene oxide-Au NPs can be immobilized for formating the sandwich immuno-complexes, which allowed for enzymatic conversion of sucrose to glucose. Thus, the concentration of MC-LR can be refelected by the converted glucose, which can be easily measured by the personal glucose meter (PGM). The PGM readout immunosensing method possessed good reproducibility and stability, which may have significant potential for other applications.
- Microcystin-LR,
- Personal glucose meter,
- Point-of-care detection,
- Graphene oxide,
- Immunoassay
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沈阳化工大学材料科学与工程学院沈阳 110142