Citation: Wu Chaoxiong, Xu Junqi, Xie Jianping, Wang Zhenyu. A streamlined high throughput screening method for the Mycobacterium neoaurum mutants with expected yield of biotransformation derivatives from sterols[J]. Chinese Chemical Letters, ;2018, 29(8): 1251-1253. doi: 10.1016/j.cclet.2018.06.025 shu

A streamlined high throughput screening method for the Mycobacterium neoaurum mutants with expected yield of biotransformation derivatives from sterols

Wu Chaoxiong ^a,1 ,
Xu Junqi ^b,1 ,
Xie Jianping ^b,, ,
Wang Zhenyu ^a,,

a.
College of Biomedical Engineering, Chongqing Medical University, Chongqing 400016, China
b.
Institute of Modern Biopharmaceuticals, School of Life Sciences, Southwest University, Chongqing 400715, China

Corresponding author: Xie Jianping, georgex@swu.edu.cn Wang Zhenyu, gdwzycq@163.com

Received Date: 24 May 2018
Revised Date: 22 June 2018
Accepted Date: 25 June 2018
Available Online: 25 August 2018

Figures(3)

Steroidal drugs have wide indications such as anti-inflammation, anti-tumor, endocrine regulation, fertility management. Phytosterol is the main starting materials for the industrial synthesis of steroid drugs. Microbial transformation of phytosterol is a simple and environmentally friendly process. Efficient microbial strains for industrial phytosterol transformation are critical for the commercial success. To this end, a 96-well plate based method was developed to discriminate the mixture of 4-androstene-3, 17-dione, androsta-1, 4-diene-3, 17-dione, and bisnoraldehyde, with different ratio of the 3 components in the mixture, which mimics the sterol bioconversion products by using the Mycobacterium neoaurum. The M. neoaurum bioconversed broth test using phytosterol as substrate also found that the spectrum methodology can evaluate the relative content of different compounds. The method is practical, high throughput and can replace the conventional HPLC-based assay for rapid selection of desired microbial strains.
- Mycobacterium neoaurum,
- Sterol,
- Biotransformation,
- Mutants,
- 4-Androstene-3, 17-dione (AD),
- Androsta-1, 4-diene-3, 17-dione (ADD)
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