Citation: Feng Ling, Chhabra Mohit, So Wing Ho, Zhu Qing, Xia Jiang, Sun Hongyan. A proximity-induced covalent fluorescent probe for selective detection of bromodomain 4[J]. Chinese Chemical Letters, ;2018, 29(7): 1147-1150. doi: 10.1016/j.cclet.2018.05.031 shu

A proximity-induced covalent fluorescent probe for selective detection of bromodomain 4

Feng Ling ^a,b ,
Chhabra Mohit ^a ,
So Wing Ho ^c ,
Zhu Qing ^d ,
Xia Jiang ^c,, ,
Sun Hongyan ^a,b,,

a.
Department of Chemistry and Center of Super-Diamond and Advanced Films(COSDAF), City University of Hong Kong, Hong Kong, China
b.
Key Laboratory of Biochip Technology, Biotech and Health Centre, Shenzhen Research Institute of City University of Hong Kong, Shenzhen 518057, China
c.
Department of Chemistry, The Chinese University of Hong Kong, Hong Kong, China
d.
College of Biotechnology and bioengineering, Zhejiang University of Technology, Hangzhou 310014, China

Corresponding author: Xia Jiang, jiangxia@cuhk.edu.hk Sun Hongyan, hongysun@cityu.edu.hk
Received Date: 9 April 2018
Revised Date: 14 May 2018
Accepted Date: 15 May 2018
Available Online: 17 July 2018

Figures(6)

Lysine acetylation is one of the most prevalent and important posttranslational modifications (PTMs) in proteins. The process can be recognized by bromodomains (BRDs), which are a class of proteininteraction modules involved in chromatin remodeling and transcriptional activation. The development of BRD fluorescent probes will be useful for monitoring the activity of BRDs in living cells as well as aiding inhibitor development. Herein we designed a peptide-based probe based on the proximity-induced protein conjugation reaction. The peptide-based probe is capable of covalently and selectively reacting with the unique cysteine residue in the bromodomain through proximity effect. Our experimental data showed that the probe displayed noticeable fluorescence response upon addition of BRD4(1). In-gel fluorescence scanning demonstrated that BRD4(1) can be covalently labelled by the probe. Moreover, the probe was shown to selectively detect BRD4(1) over other proteins. We envision that the probe developed in this study will provide a useful tool to further investigate the biological roles of BRDs.
- Peptide-based probe,
- Bromodomain,
- Fluorescent probe,
- Proximity effect,
- Peptides
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