Citation: Liu Jinrong, Dong Suwei. Synthetic studies toward human interleukin-5[J]. Chinese Chemical Letters, ;2018, 29(7): 1131-1134. doi: 10.1016/j.cclet.2018.05.014 shu

Synthetic studies toward human interleukin-5

Liu Jinrong ,
Dong Suwei ^,

State Key Laboratory of Natural and Biomimetic Drugs, Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing 100191, China

Corresponding author: Dong Suwei, dongs@hsc.pku.edu.cn
Received Date: 24 March 2018
Revised Date: 24 April 2018
Accepted Date: 28 April 2018
Available Online: 7 July 2018

Figures(5)

Interleukin 5 (IL-5) is a human cytokine that regulates eosinophil growth and activation, and it plays essential roles in diseases associated with increased level of eosinophils such as asthma. Many studies have been conducted on this important glycoprotein, but all of them utilized recombinantly expressed samples. Here we describe our attempted chemical synthesis of IL-5, using protocols of Fmoc-SPPS and peptidyl hydrazide-based native chemical ligation, where the disulfide-reduced form of IL-5 is assembled from three peptide segments. Reconstitution of the protein under different folding conditions has also been investigated.
- Interleukin 5,
- Eosinophilic disorders,
- Asthma,
- Solid phase peptide synthesis,
- Native chemical ligation,
- Mirror-image phage display
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