Citation: Lin Shaomin, He Chunmao. A mini-review on the enzyme-mediated manipulation of proteins/peptides[J]. Chinese Chemical Letters, ;2018, 29(7): 1017-1021. doi: 10.1016/j.cclet.2018.05.006 shu

A mini-review on the enzyme-mediated manipulation of proteins/peptides

Lin Shaomin ,
He Chunmao ^,

School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou 510640, China

Corresponding author: He Chunmao, hecm@scut.edu.cn
Received Date: 14 April 2018
Revised Date: 26 April 2018
Accepted Date: 28 April 2018
Available Online: 3 July 2018

Figures(5)

Recent years have seen an ever increasing number of enzyme mediated protein/peptide modification reactions, which contribute significantly to the elucidation of related biological functions. The many available enzymes have, however, caused difficulties for practitioners in choosing the most appropriate enzyme for a certain purpose. This review surveyed the widely used enzymes (i.e., sortases, butelase 1, subtiligase, formylglycine generating enzyme and farnesyltransferase) in the manipulation of proteins/peptides, and the application fields of these enzymes as well as the advantages and limitations of each enzyme are summarized.
- Protein modification,
- Protein labeling,
- Protein ligation,
- Site-selective,
- Enzyme-mediated
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沈阳化工大学材料科学与工程学院沈阳 110142