Citation: Kai Ming, Zhang Wei, Xie Huan, Liu Liwei, Huang Sujie, Li Xiao, Zhang Zhengzheng, Liu Yuyang, Zhang Bangzhi, Song Jingjing, Wang Rui. Effects of linker amino acids on the potency and selectivity of dimeric antimicrobial peptides[J]. Chinese Chemical Letters, ;2018, 29(7): 1163-1166. doi: 10.1016/j.cclet.2018.04.011 shu

Effects of linker amino acids on the potency and selectivity of dimeric antimicrobial peptides

Kai Ming ^a ,
Zhang Wei ^b ,
Xie Huan ^b ,
Liu Liwei ^a ,
Huang Sujie ^a ,
Li Xiao ^a ,
Zhang Zhengzheng ^b ,
Liu Yuyang ^b ,
Zhang Bangzhi ^a ,
Song Jingjing ^b,, ,
Wang Rui ^b,,

1.
School of Life Sciences, Lanzhou University, Lanzhou 730000, China
2.
Laboratory of Preclinical Study for New Drugs of Gansu Province, School of Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China

Corresponding author: Song Jingjing, songjj@lzu.edu.cn Wang Rui, wangrui@lzu.edu.cn
Received Date: 6 March 2018
Revised Date: 25 March 2018
Accepted Date: 8 April 2018
Available Online: 14 July 2018

Figures(3)

Dimerization is an effective strategy for designing antimicrobial peptides that combine the advantages of different native peptides. In this study, we explored the effects of different linker amino acids, including leucine, proline and aminocaproic acid, on the anticancer, antimicrobial and hemolytic activities of the heteromeric antimicrobial peptides AM-1, AM-2, and AM-3. Proline and aminocaproic acid are ideal linkers for increasing the potency and selectivity of heteromeric antimicrobial peptides. The results of MD simulations provided a rationalization for this observation. Both AM-2, which had a proline linker, and AM-3, which had an aminocaproic acid linker, adopted a compact conformation in water and a bent conformation in membranes. This change in the flexible structures of AM-2 and AM-3 could have resulted in decreased binding of these peptides to zwitterionic lipid bilayers and increased damage to mixed lipid bilayers containing acidic phospholipids. In short, these findings obtained via assessing the effects of linker amino acids will contribute to the design of ideal heteromeric antimicrobial peptides with high selectivity and potency.
- Linker amino acid,
- Flexibility,
- Heteromeric antimicrobial peptides,
- Potency and selectivity
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1.
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