Citation: Zhu Tong, Song Lu, Li Ruifeng, Wu Bian. Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase[J]. Chinese Chemical Letters, ;2018, 29(7): 1116-1118. doi: 10.1016/j.cclet.2018.03.033 shu

Enzymatic clickable functionalization of peptides via computationally engineered peptide amidase

Zhu Tong ^a,b,1 ,
Song Lu ^a,c,1 ,
Li Ruifeng ^a,b ,
Wu Bian ^a,,

a.
CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China
b.
University of Chinese Academy of Sciences, Beijing 100101, China
c.
State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing 100101, China

Corresponding author: Wu Bian, wub@im.ac.cn

Received Date: 5 February 2018
Revised Date: 21 March 2018
Accepted Date: 23 March 2018
Available Online: 30 July 2018

Figures(2)

Directed peptides C-terminal modification enabled by the engineered biomolecular catalyst-peptide amidase 12B has been achieved via computational protein engineering. The engineered enzyme exhibits great promising potential in the C-terminal modification of opioid peptides using prop-2-yn-1-amine (PYA) or prop-2-en-1-amine (PEA) as the nucleophile. A variety of opioid peptides could be readily functionalized at the C-terminal chain in high yield in a mild and selective manner. Notably, modified opioid peptides bearing alkynyl moiety could be further functionalized through well-established click reaction.
- Peptide amidase,
- Opioid peptides,
- C-terminal modification,
- Click reaction,
- Functionalization
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