Citation: Yang Beibei, Liu Dongmei, Zhu Lina, Liu Yun, Wang Xiaoning, Qiao Liang, Zhang Weijia, Liu Baohong. Sensitive detection of thyroid stimulating hormone by inkjet printed microchip with a double signal amplification strategy[J]. Chinese Chemical Letters, ;2018, 29(12): 1879-1882. doi: 10.1016/j.cclet.2018.01.042 shu

Sensitive detection of thyroid stimulating hormone by inkjet printed microchip with a double signal amplification strategy

Yang Beibei ^a ,
Liu Dongmei ^b ,
Zhu Lina ^a ,
Liu Yun ^a ,
Wang Xiaoning ^b,*, ,
Qiao Liang ^a ,
Zhang Weijia ^a ,
Liu Baohong ^a,*,

a.
Department of Chemistry, Shanghai Stomatological Hospital, Collaborative Innovation Center of Chemistry for Energy Materials, Fudan University, Shanghai 200433, China
b.
Department of Pharmacy, Qingdao Municipal Hospital, Qingdao 266001, China

15563909510@163.com

bhliu@fudan.edu.cn

Received Date: 3 December 2017
Revised Date: 5 January 2018
Accepted Date: 23 January 2018
Available Online: 31 December 2018

Figures(4)

An electrochemical immunosensor for sensitive detection of thyroid stimulating hormone (TSH) has been developed by using an inkjet printed microchip and based on a double signal amplification strategy using magnetic beads (MBs), alkaline phosphatase (ALP) and p-aminophenyl phosphate (pAPP) reaction. Differential pulse voltammetry (DPV), cyclic voltammogram (CV) and amperometric i-t curve (i-t) were employed to characterize the immunosensor. High sensitivity and good selectivity were observed. The detection linear range was from 0.01 μIU/mL to 10 μIU/mL, in which the peak currents increased along with the concentration. The detection limit was 0.005 μIU/mL at S/N=3. The immunosensor was also applied for TSH detection in human serum with recoveries from 98.0% to 101.8% and relative standard deviations from 1.3% to 3.1%, demonstrating potential value in clinical diagnosis.
- Electrochemical immunosensor,
- Inkjet printed microchip,
- Signal amplification,
- Thyroid stimulating hormone,
- Alkaline phosphatase
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