Citation: Zhang Lin, Xu Tengfei, Pi Zifeng, Zheng Meizhu, Song Fengrui, Liu Zhiqiang. Effects of the ambient fine particulate matter (PM2.5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS[J]. Chinese Chemical Letters, ;2019, 30(1): 90-94. doi: 10.1016/j.cclet.2017.11.019 shu

Effects of the ambient fine particulate matter (PM2.5) exposure on urinary metabolic profiles in rats using UPLC-Q-TOF-MS

Zhang Lin ^a,b ,
Xu Tengfei ^a ,
Pi Zifeng ^a,, ,
Zheng Meizhu ^c ,
Song Fengrui ^a ,
Liu Zhiqiang ^a,,

a.
Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun Center of Mass Spectrometry, Key Laboratory of Chinese Medicine Chemistry and Mass Spectrometry, Changchun 130022, China
b.
School of Pharmaceutical Sciences, Jilin University, Changchun 130021, China
c.
The Central Laboratory, Changchun Normal University, Changchun 130032, China

Corresponding author: Pi Zifeng, mslab21@ciac.ac.cn Liu Zhiqiang, liuzq@ciac.ac.cn
Received Date: 26 September 2017
Revised Date: 10 November 2017
Accepted Date: 14 November 2017
Available Online: 16 January 2017

Figures(3)

In order to study the damage of ambient fine particulate matter (PM2.5) to organism in the environment, the method of urinary metabolomics was used to detect the potential endogenous metabolites and their related metabolic pathways to clarify the toxicity mechanisms. Particles of PM2.5 collected from the fibrous membrane were extracted ultrasonically and dissolved in physiological saline to prepare PM2.5 suspension. The solution of saline and fine particular matter was injected into the trachea of rats respectively. The rats were injected two times a week for four weeks. Samples of 24-h urine were collected at the 14th day after the end of exposure, and the ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS) was used for the metabolomics detection. Principal component analysis (PCA) was used to investigate the global metabolomic alterations and a clear separation in the scatter diagram were observed. 17 potential endogenous metablites were identified from urinary samples in rats by UPLC-Q-TOF-MS. The primary metabolism pathways involved pentose and glucuronate interconversions, starch and sucrose metabolism, tryptophan metabolism, tyrosine metabolism, phenylalanine metabolism, purine metabolism, acetaminophen metabolism pathway, retinol metabolism and valproic acid metabolism pathway. The results are helpful to understand the toxicological mechanisms of PM2.5 and screened out potential biomarkers of rats which exposure to PM2.5.
- Ambient fine particulate matter,
- UPLC-Q-TOF-MS,
- Metabolomics,
- Urine
1. [1]
  C.A. Pope, R.T. Burnett, M.J. Thun, et al., J. Am. Med. Assoc. 287(2002) 1132-1141. doi: 10.1001/jama.287.9.1132
2. [2]
  D. Hartono, B. Lioe, Y. Zhang, B. Li, et al., Sci. Rep. 7(2017) 644. doi: 10.1038/s41598-017-00449-5
3. [3]
  Y.L. Shi, L.H. Deng, J. Med. Bio. 28(2013) 127-134.
4. [4]
  Y.Q. Lu, Y.T. Yuan, J. Anhui. Agric. 41(2013) 13988-13989.
5. [5]
  S. Barnesa, H.P. Bentonj, K. Casazzac, et al., J. Mass Spectrom. 51(2016) 461-475. doi: 10.1002/jms.3782
6. [6]
  N. Psychogios, D.D. Hau, J. Peng, et al., PLoS. One 6(2011) e16957. doi: 10.1371/journal.pone.0016957
7. [7]
  O. Beckonert, H.C. Keun, T.M.D. Ebbels, et al., Nat. Protoc. 2(2007) 2692. doi: 10.1038/nprot.2007.376
8. [8]
  Z.H. Su, S.Q. Li, G.A. Zou, C.Y. Yu, et al., J. Pharm. Bio. Anal. 55(2011) 533-539. doi: 10.1016/j.jpba.2011.02.013
9. [9]
  D.Pawlak, A.Tankiewicz, T.Matys, etal., J.Physiol.Pharmacol.54(2003)175-189.
10. [10]
  D. Pawlak, A. Tankiewicz, W. Buczko, J. Physiol. Pharmacol. 52(2001) 755-766.
11. [11]
  D.H. Wang, S.M. Wang, L. Wei, et al., Adv. Physiol. Sci. 47(2016) 43-46.
12. [12]
  L.E. Goldstein, M.C. Leopold, X. Huang, et al., Biochemistry 39(2000) 7266-7275. doi: 10.1021/bi992997s
13. [13]
  S. Okuda, N. Nishiyama, H. Saito, et al., J. Neurochem. 70(1998) 299-307.
14. [14]
  E.P. Wang, Y. Sun, H. Yue, et al., Chin. J. Anal. Chem. 44(2016) 1410-1418.
15. [15]
  N.G. Buckman, J.O. Hill, R.J. Magee, et al., J. Chromatogr. A 284(1984) 441-446. doi: 10.1016/S0021-9673(01)87845-6
16. [16]
  G. Glorieux, R. Vanholder, Contrib. Nephrol. 168(2011) 117-128.
17. [17]
  T. Miyagaki, I. Aoyama, M. Ise, et al., Nephrol. Dial. Transpl. 15(2000) 1773-1781. doi: 10.1093/ndt/15.11.1773
18. [18]
  E.J. Want, I.D. Wilson, H. Gika, et al., Nat. Protoc. 5(2010) 1005-1018. doi: 10.1038/nprot.2010.50
19. [19]
  N.S. Jain, U.H.N. Dürr, A. Ramamoorthy, Chin. Chem. Lett. 26(2015) 407-415. doi: 10.1016/j.cclet.2015.03.001
1. [1]
  Cheng Guo , Xiaoxiao Zhang , Xiujuan Hong , Yiqiu Hu , Lingna Mao , Kezhi Jiang . Graphene as adsorbent for highly efficient extraction of modified nucleosides in urine prior to liquid chromatography-tandem mass spectrometry analysis. Chinese Chemical Letters, 2024, 35(4): 108867-. doi: 10.1016/j.cclet.2023.108867
2. [2]
  Xiaoxiao Wang , Bolun Wang , Fenfen Ji , Jie Yan , Jiacheng Fang , Doudou Zhang , Ji Xu , Jing Ji , Xinran Hao , Hemi Luan , Yanjun Hong , Shulan Qiu , Min Li , Zhu Yang , Wenlan Liu , Xiaodong Cai , Zongwei Cai . Discovery of plasma biomarkers for Parkinson’s disease diagnoses based on metabolomics and lipidomics. Chinese Chemical Letters, 2024, 35(11): 109653-. doi: 10.1016/j.cclet.2024.109653
3. [3]
  Yanhua Chen , Xian Ding , Jun Zhou , Zhaoying Wang , Yunhai Bo , Ying Hu , Qingce Zang , Jing Xu , Ruiping Zhang , Jiuming He , Fen Yang , Zeper Abliz . Plasma metabolomics combined with mass spectrometry imaging reveals crosstalk between tumor and plasma in gastric cancer genesis and metastasis. Chinese Chemical Letters, 2025, 36(1): 110351-. doi: 10.1016/j.cclet.2024.110351
4. [4]
  Xuyang Chai , Hiu-Lok Ngan , Yuanyuan Song , Zenghua Qi , Lifang Zhao , Wenqi Chen , Shanshan Chen , Zhenhua Yang , Ruijin Li , Chuan Dong , Zhu Yang , Zongwei Cai . Real-world PM_2.5 exposure induces prostaglandin disruption and low-density lipoprotein oxidation, exacerbating atherogenesis in ApoE^−/− mice. Chinese Chemical Letters, 2025, 36(9): 110671-. doi: 10.1016/j.cclet.2024.110671
5. [5]
  Qiongqiong Wan , Zhourui Zhang , Mengmeng Zhao , Xianqin Ruan , Yanhong Hao , Jiajun Deng , Yunlang She , Minglei Yang , Yongxiang Song , Feng Jin , Ailin Wei , Sheng Zhong , Jie Zheng , Dong Xie , Suming Chen . Efficient diagnosis of benign and malignant pulmonary nodules based on Nano-zero-valent iron enhanced serum metabolic fingerprinting. Chinese Chemical Letters, 2025, 36(10): 110794-. doi: 10.1016/j.cclet.2024.110794
6. [6]
  Qian Wu , Yuanxia Lv , Zixuan Guo , Zhihao Zhao , Zhimin Zhang , Hongmei Lu . A Case Study and Practice of Research-Oriented Comprehensive Instrumental Analysis Laboratory Courses. University Chemistry, 2025, 40(10): 194-202. doi: 10.12461/PKU.DXHX202411063
7. [7]
  Aolei Tan , Xiaoxiao Ma . Exploring the functional roles of small-molecule metabolites in disease research: Recent advancements in metabolomics. Chinese Chemical Letters, 2024, 35(8): 109276-. doi: 10.1016/j.cclet.2023.109276
8. [8]
  Yue Xu , Lingzhi Wang , Liu Yang , Renliang Xue , Haowen Zhu , Qifeng Liu , Xueqi Lv , Ruiping Zhang , Jun Tu , Qingce Zang , Yinghong Wang . Spatial metabolomics combined with transcriptomics to reveal heterogeneous metabolism and drug response in the heart of rats with heart failure. Chinese Chemical Letters, 2025, 36(7): 110958-. doi: 10.1016/j.cclet.2025.110958
9. [9]
  Yao-Hua Gu , Yu Chen , Qing Li , Neng-Bin Xie , Xue Xing , Jun Xiong , Min Hu , Tian-Zhou Li , Ke-Yu Yuan , Yu Liu , Tang Tang , Fan He , Bi-Feng Yuan . Metabolome profiling by widely-targeted metabolomics and biomarker panel selection using machine-learning for patients in different stages of chronic kidney disease. Chinese Chemical Letters, 2024, 35(11): 109627-. doi: 10.1016/j.cclet.2024.109627
10. [10]
  Xinyue Han , Yunhan Yang , Jiayin Lu , Yuxiang Lin , Dongxue Zhang , Ling Lin , Liang Qiao . Efficient serum lipids profiling by TiO₂-dopamin-assisted MALDI-TOF MS for breast cancer detection. Chinese Chemical Letters, 2025, 36(5): 110183-. doi: 10.1016/j.cclet.2024.110183
11. [11]
  Wantong Zhang , Zixing Xu , Guofei Dai , Zhijian Li , Chunhui Deng . Removal of Microcystin-LR in lake water sample by hydrophilic mesoporous silica composites under high-throughput MALDI-TOF MS detection platform. Chinese Chemical Letters, 2024, 35(5): 109135-. doi: 10.1016/j.cclet.2023.109135
12. [12]
  Yuchen Zhang , Lifeng Ding , Zhenghe Xie , Xin Zhang , Xiaofeng Sui , Jian-Rong Li . Porous sorbents for direct capture of carbon dioxide from ambient air. Chinese Chemical Letters, 2025, 36(3): 109676-. doi: 10.1016/j.cclet.2024.109676
13. [13]
  Yue Zhang , Xiaoya Fan , Xun He , Tingyu Yan , Yongchao Yao , Dongdong Zheng , Jingxiang Zhao , Qinghai Cai , Qian Liu , Luming Li , Wei Chu , Shengjun Sun , Xuping Sun . Ambient electrosynthesis of urea from carbon dioxide and nitrate over Mo₂C nanosheet. Chinese Chemical Letters, 2024, 35(8): 109806-. doi: 10.1016/j.cclet.2024.109806
14. [14]
  Chao-Long Chen , Rong Chen , La-Sheng Long , Lan-Sun Zheng , Xiang-Jian Kong . Anchoring heterometallic cluster on P-doped carbon nitride for efficient photocatalytic nitrogen fixation in water and air ambient. Chinese Chemical Letters, 2024, 35(4): 108795-. doi: 10.1016/j.cclet.2023.108795
15. [15]
  Jing Wang , Zenghui Li , Xiaoyang Liu , Bochao Su , Honghong Gong , Chao Feng , Guoping Li , Gang He , Bin Rao . Fine-tuning redox ability of arylene-bridged bis(benzimidazolium) for electrochromism and visible-light photocatalysis. Chinese Chemical Letters, 2024, 35(9): 109473-. doi: 10.1016/j.cclet.2023.109473
16. [16]
  Shuli Wang , Xuemin Kong , Siting Cai , Yunshu Luo , Yuxuan Gu , Xiaotong Fan , Guolong Chen , Xiao Yang , Zhong Chen , Yue Lin . Solvent engineering in perovskite nanocrystal colloid inks for super-fine electrohydrodynamic inkjet printing of color conversion microstructures in micro-LED displays. Chinese Chemical Letters, 2025, 36(8): 110976-. doi: 10.1016/j.cclet.2025.110976
17. [17]
  Jianfang Cao , Xue Ma , Xinyu Chen , Tianci Zhang , Wen Sun . Theoretical perspective on fine-tuning ISC efficiency in D-A BODIPY photosensitizers through heteroatom incorporation and nitrogen ring strain modulation. Chinese Chemical Letters, 2025, 36(10): 110897-. doi: 10.1016/j.cclet.2025.110897
18. [18]
  Jinqi Yang , Xiaoxiang Hu , Yuanyuan Zhang , Lingyu Zhao , Chunlin Yue , Yuan Cao , Yangyang Zhang , Zhenwen Zhao . Direct observation of natural products bound to protein based on UHPLC-ESI-MS combined with molecular dynamics simulation. Chinese Chemical Letters, 2025, 36(5): 110128-. doi: 10.1016/j.cclet.2024.110128
19. [19]
  Guo-Ping Yin , Ya-Juan Li , Li Zhang , Ling-Gao Zeng , Xue-Mei Liu , Chang-Hua Hu . Citrinsorbicillin A, a novel homotrimeric sorbicillinoid isolated by LC-MS-guided with cytotoxic activity from the fungus Trichoderma citrinoviride HT-9. Chinese Chemical Letters, 2024, 35(8): 109035-. doi: 10.1016/j.cclet.2023.109035
20. [20]
  Pengyu Chen , Beibei Chen , Man He , Yuxi Zhou , Lei Lei , Jian Han , Bingsheng Zhou , Ligang Hu , Bin Hu . Nanoplastics and nano-ZnO facilitate Cd accumulation in zebrafish larvae via a distinct pathway: Revelation by LA-ICP-MS imaging. Chinese Chemical Letters, 2025, 36(2): 109908-. doi: 10.1016/j.cclet.2024.109908

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