Citation: Guo Lingling, Wang Ting, Chen Zhan, He Nongyue, Chen Yaozhong, Yuan Tao. Light scattering based analyses of the effects of bovine serum proteins on interactions of magnetite spherical particles with cells[J]. Chinese Chemical Letters, ;2018, 29(8): 1291-1295. doi: 10.1016/j.cclet.2017.11.017 shu

Light scattering based analyses of the effects of bovine serum proteins on interactions of magnetite spherical particles with cells

Guo Lingling ^a,1 ,
Wang Ting ^a,1,, ,
Chen Zhan ^b ,
He Nongyue ^a,, ,
Chen Yaozhong ^a ,
Yuan Tao ^c

a.
State Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
b.
Department of Chemistry, University of Michigan, Ann Arbor MI 48109, United States
c.
Department of Orthopedic, Nanjing Jinling Hospital, Nanjing 210096, China

Corresponding author: Wang Ting, tingwang@seu.edu.cn He Nongyue, nyhe1958@163.com

Received Date: 27 September 2017
Revised Date: 26 October 2017
Accepted Date: 14 November 2017
Available Online: 16 August 2017

Figures(5)

Information concerns endocytosis and autophagic effects of magnetite particles is crucial for understanding the particle-cell interactions. In this work, we investigated the effects of bovine serum proteins on the endocytosis of magnetite spherical particles (MSPs). Autophagic effects of MSPs in breast cancer cells were studied. Light scattering based flow cytometry and microscopy were used for evaluating the uptake potential of MSPs by cells and the cellular autophagosome accumulation. Results showed bovine serum proteins significantly reduced the endocytosis of MSPs by decreasing their adsorption to cell membranes. Additionally, serum proteins had influences on the endocytic mechanisms of MSPs. Autophagosome accumulation could be caused by the internalized MSPs rather than the particles associated with cell membrane. Above fundamental findings promote our understandings upon the interactions of MSPs with cells. Light scattering based methods were proved to be simple and effective. The present work may promote their application in studies upon endocytosis of metallic particles in the future.
- Light scattering,
- Endocytosis,
- Autophagic effects,
- Autophagosome,
- Magnetite spherical particles
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