Citation: Chen Chaohui, Ji Xinghu. A dual-amplification system for colorimetric DNA detection based on the assembly of biomolecules[J]. Chinese Chemical Letters, ;2018, 29(8): 1287-1290. doi: 10.1016/j.cclet.2017.10.013 shu

A dual-amplification system for colorimetric DNA detection based on the assembly of biomolecules

Chen Chaohui ^a,b ,
Ji Xinghu ^b,,

a.
Institute for Interdisciplinary Research, Jianghan University, Wuhan 430056, China
b.
Key Laboratory of Analytical Chemistry for Biology and Medicine, Ministry of Education, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China

Corresponding author: Ji Xinghu, xhu@whu.edu.cn
Received Date: 3 August 2017
Revised Date: 9 September 2017
Accepted Date: 12 October 2017
Available Online: 13 August 2017

Figures(4)

A dual-amplification system is reported to apply in DNA sensing via the assembly of DNA and protein. In this process, the biotinylatedcapature DNA bounded with streptavidin (SA) through the biotinstreptavidin reaction, and then the assembly of DNA and protein was triggered by the linker DNA after the target hybridized with biotinylatedcapature DNA. Sequentially, the 3, 3', 5, 5'-tetramethylbenzidine (TMB) was oxidized by H₂O₂ under the catalysis of horseradish peroxidase. Based on the variation of the color and the UV-vis absorbance intensities, qualitative and quantitative DNA analyses were realized. This proposed method could detect the target DNA as low as 1.75 pmol/L and discriminate perfectly matched target DNA from the mismatch DNA. What's more, it can be expanded to detect other molecules with a reasonable design of the corresponding DNA sequences.
- Dual-amplification,
- Colorimetric,
- DNA detection,
- Self-assembly,
- Biosensor
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沈阳化工大学材料科学与工程学院沈阳 110142