Citation: Zhao Chun-Ling, Hua Mei, Yang Can-Yu, Yang Yun-Hui. A novel aptasensor based on 3D-inorganic hybrid composite as immobilized substrate for sensitive detection of platelet-derived growth factor[J]. Chinese Chemical Letters, ;2017, 28(7): 1417-1423. doi: 10.1016/j.cclet.2017.02.010 shu

A novel aptasensor based on 3D-inorganic hybrid composite as immobilized substrate for sensitive detection of platelet-derived growth factor

College of Chemistry and Chemical Engineering, Yunnan Normal University, Kunming 650092, China

Corresponding author: Yang Yun-Hui, yyhui2002@aliyun.com
Received Date: 9 November 2016
Revised Date: 14 January 2017
Accepted Date: 6 February 2017
Available Online: 24 July 2017

Figures(7)

A novel electrochemical detection approach for platelet-derived growth factor (PDGF) via "sandwich" structure is reported in this paper. 3D-4MgCO₃·Mg(OH)₂·4H₂O-Au NPs inorganic hybrid composite was utilized as immobilized substrate for sensitive PDGF detection and Pt-Au bimetallic nanoparticles were labelled on PDGF aptamer to indirectly detect PDGF for the first time. The proposed aptasensor exhibited a high catalytic efficiency towards reduction of H₂O₂, hence the sensitive detection of PDGF was achieved. Results showed that the aptasensor exhibited excellent linear response to PDGF, in the range of 0.1 pg/mL-10 ng/mL (4 fmol/L-400 pmol/L), with detection limit of 0.03 pg/mL (1.2 fmol/L).
- Aptasensor,
- Platelet-derived growth factor,
- 3D-inorganic hybrid composite,
- Pt-Au bimetallic nanoparticles,
- 4MgCO₃·Mg(OH)₂·4H₂O-Au NPs
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