Citation: Wang Xiao-Dong, Liu Yu-Jie, Li Fen-Jie, Li Zhi-Li. Poplar catkin: A natural biomaterial for highly specific and efficient enrichment of sialoglycopeptides[J]. Chinese Chemical Letters, ;2017, 28(5): 1018-1026. doi: 10.1016/j.cclet.2017.02.001 shu

Poplar catkin: A natural biomaterial for highly specific and efficient enrichment of sialoglycopeptides

Department of Biophysics and Structural Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & School of Basic Medicine, Peking Union Medical College, Beijing 100005, China

Corresponding author: Li Zhi-Li, lizhili@ibms.pumc.edu.cn

Received Date: 28 October 2016
Revised Date: 30 November 2016
Accepted Date: 23 December 2016
Available Online: 4 May 2017

Figures(9)

Sialic acids as terminal entities of larger glycans linked to proteins and lipids are involved in multiple different pathological and physiological processes. Structural characterisation of sialoglycoconjugates is required to understand their biological function. However, a comprehensive sialylation analysis of sialoglycoconjugates has remained challenges. In this study, we employ a natural biomaterial, poplar catkin derived from white poplar tree (Populus tomentosa Carr.), to develop a novel capturing microtip for selective and efficient enrichment of sialoglycopeptides, without losses of sialic acid residues and water molecules from sialoglycopeptides. Scanning electron microscopy and Fourier-transform infrared spectroscopy analysis, along with Mäule and Wiesner staining assays, indicated that the main components on the outer layer of the poplar catkin are syringyl and guaiacyl lignins which play a key role in enriching sialoglycopeptides from complex peptide mixture.
- Sialogycopeptide,
- Poplar catkin,
- Enrichment,
- Natural biomaterial,
- Mass spectrometry
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