Citation: Chen Shuang-Shuang, Lu Xue-Min, Lu Qing-Hua. Effects of concave and convex substrate curvature on cell mechanics and the cytoskeleton[J]. Chinese Chemical Letters, ;2017, 28(4): 818-826. doi: 10.1016/j.cclet.2016.10.039 shu

Effects of concave and convex substrate curvature on cell mechanics and the cytoskeleton

School of Chemistry and Chemical Engineering, State Key Laboratory of Metal Matrix Composites, Shanghai Jiao Tong University, Shanghai 200240, China

Corresponding author: Lu Qing-Hua, qhlu@sjtu.edu.cn
Received Date: 13 September 2016
Revised Date: 18 October 2016
Accepted Date: 24 October 2016
Available Online: 2 April 2016

Figures(9)

In order to understand how cells respond to concave and convex subcellular surface structures, colloidal crystal array and honeycomb-structured surfaces composed of highly ordered hexagonal units with completely inverse curvature were fabricated via facile self-assembly and breath figure approaches, respectively.The influence of hexagonal surface curvature on cell fate was subsequently investigated. Cells underwent more extensive spreading on the convex colloidal crystal array surface, while adhesive forces were higher on the concave honeycomb surface.The behaviors of cells on the different surfaces were investigated by comparing cell morphology, cellular adhesive force and cytoskeleton structure.The results revealed comprehensive differences in cell behavior between those on concave honeycomb surfaces and convex colloidal crystal arrays.
- Colloidal crystal array,
- Honeycomb structure,
- Substrate curvature,
- Cytoskeleton,
- Adhesive force
1. [1]
  Yeung T., Georges P.C., Flanagan L.A.. Effects of substrate stiffness on cell morphology cytoskeletal structure, and adhesion[J]. Cell Motil.Cytoskeleton, 2005,60:24-34. doi: 10.1002/(ISSN)1097-0169
2. [2]
  Mullen C.A., Vaughan T.J., Voisin M.C.. Cell morphology and focal adhesion location alters internal cell stress[J]. J.R.Soc.Interface, 2014,1120140885. doi: 10.1098/rsif.2014.0885
3. [3]
  Keselowsky B.G., Collard D.M., García A.J.. Surface chemistry modulates fibronectin conformation and directs integrin binding and specificity to control cell adhesion[J]. J.Biomed.Mater.Res.A, 2003,66A:247-259. doi: 10.1002/jbm.a.v66a:2
4. [4]
  Gibson L.J., Ashby M.F. The mechanics of three-dimensional cellular materials[J]. Proc.R.Soc.A, 1982,382:43-59. doi: 10.1098/rspa.1982.0088
5. [5]
  Chen J., Irianto J., Inamdar S.. Cell mechanics structure, and function are regulated by the stiffness of the three-dimensional microenvironment[J]. Biophys.J., 2012,103:1188-1197. doi: 10.1016/j.bpj.2012.07.054
6. [6]
  Bao G., Suresh S. Cell and molecular mechanics of biological materials[J]. Nat. Mater., 2003,2:715-725. doi: 10.1038/nmat1001
7. [7]
  Tan J., Saltzman W.M. Biomaterials with hierarchically defined micro-and nanoscale structure[J]. Biomaterials, 2004,25:3593-3601. doi: 10.1016/j.biomaterials.2003.10.034
8. [8]
  Ranella A., Barberoglou M., Bakogianni S., Fotakis C., Stratakis E. Tuning cell adhesion by controlling the roughness and wettability of 3D micro/nano silicon structures[J]. Acta Biomater., 2010,6:2711-2720. doi: 10.1016/j.actbio.2010.01.016
9. [9]
  Zhao L.Z., Mei S.L., Chu P.K., Zhang Y.M., Wu Z.F. The influence of hierarchical hybrid micro/nano-textured titanium surface with titania nanotubes on osteoblast functions[J]. Biomaterials, 2010,31:5072-5082. doi: 10.1016/j.biomaterials.2010.03.014
10. [10]
  Chen C.S., Mrksich M., Huang S., Whitesides G.M., Ingber D.E. Geometric control of cell life and death[J]. Science, 1997,276:1425-1428. doi: 10.1126/science.276.5317.1425
11. [11]
  Newhart A., Janicki S.M. Seeing is believing:visualizing transcriptional dynamics in single cells[J]. J.Cell.Physiol., 2014,229:259-265. doi: 10.1002/jcp.24445
12. [12]
  Dubey G.P., Ben-Yehuda S.. Intercellular nanotubes mediate bacterial communication[J]. Cell, 2011,144:590-600. doi: 10.1016/j.cell.2011.01.015
13. [13]
  Fletcher D.A., Mullins D. Cell mechanics and the cytoskeleton[J]. Nature, 2010,463:485-492. doi: 10.1038/nature08908
14. [14]
  Yamaki K., Harada I., Goto M., Cho C.S., Akaike T. Regulation of cellular morphology using temperature-responsive hydrogel for integrin-mediated mechanical force stimulation[J]. Biomaterials, 2009,30:1421-1427. doi: 10.1016/j.biomaterials.2008.11.036
15. [15]
  Chen L., Liu X.L., Su B.. Aptamer-mediated efficient capture and release of T lymphocytes on nanostructured surfaces[J]. Adv.Mater., 2011,23:4376-4380. doi: 10.1002/adma.201102435
16. [16]
  Frame M.D., Sarelius I.H. Flow-induced cytoskeletal changes in endothelial cells growing on curved surfaces[J]. Microcirculation, 2000,7:419-427. doi: 10.1111/micc.2000.7.issue-6
17. [17]
  J. A. Sanz-Herrera, P. Moreo, J. M. García-Aznar, M. Doblaré, On the effect of substrate curvature on cell mechanics, Biomaterials 30(2009)6674-6686.
18. [18]
  James J., Goluch E.D., Hu H., Liu C., Mrksich M. Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity[J]. Cell Motil.Cytoskeleton, 2008,65:841-852. doi: 10.1002/cm.v65:11
19. [19]
  Tamura M. Inhibition of cell migration, spreading, and focal adhesions by tumor suppressor PTEN[J]. Science, 1998,280:1614-1617. doi: 10.1126/science.280.5369.1614
20. [20]
  Kim S.V., Mehal W.Z., Dong X.M.. Modulation of cell adhesion and motility in the immune system by Myo1f[J]. Science, 2006,314:136-139. doi: 10.1126/science.1131920
21. [21]
  Cukierman E., Pankov R., Stevens D.R., Yamada K.M. Taking cell-matrix adhesions to the third dimension[J]. Science, 2001,294:1708-1712. doi: 10.1126/science.1064829
22. [22]
  Raic A., Rödling L., Kalbacher H., Lee-Thedieck C.. Biomimetic macroporous PEG hydrogels as 3D scaffolds for the multiplication of human hematopoietic stem and progenitor cells[J]. Biomaterials, 2014,35:929-940. doi: 10.1016/j.biomaterials.2013.10.038
23. [23]
  Park J., Babensee J.E. Differential functional effects of biomaterials on dendritic cell maturation[J]. Acta Biomater., 2012,8:3606-3617. doi: 10.1016/j.actbio.2012.06.006
24. [24]
  A. J. García, Get a grip: integrins in cell-biomaterial interactions, Biomaterials 26(2005)7525-7529.
25. [25]
  Whitesides G.M., Grzybowski B. Self-assembly at all scales[J]. Science, 2002,295:2418-2421. doi: 10.1126/science.1070821
26. [26]
  Xu Y.X., Sheng K.X., Li C., Shi G.Q. Self-assembled graphene hydrogel via a one-step hydrothermal process[J]. ACS Nano, 2010,4:4324-4330. doi: 10.1021/nn101187z
27. [27]
  Zhang S.Y., Regulacio M.D., Han M.Y. Self-assembly of colloidal one-dimensional nanocrystals[J]. Chem.Soc.Rev., 2014,43:2301-2323. doi: 10.1039/c3cs60397k
28. [28]
  Yamazaki H., Gotou S., Ito K.. Micropatterned culture of HepG2 spheroids using microwell chip with honeycomb-patterned polymer film[J]. J.Biosci. Bioeng., 2014,118:455-460. doi: 10.1016/j.jbiosc.2014.03.006
29. [29]
  A.S.de León, J.Rodríguez-Hernández , Cortajarena A.L. Honeycomb patterned surfaces functionalized with polypeptide sequences for recognition and selective bacterial adhesion[J]. Biomaterials, 2013,34:1453-1460. doi: 10.1016/j.biomaterials.2012.10.074
30. [30]
  Zhu Y.D., Sheng R.L., Luo T.. Honeycomb-structured films by multifunctional amphiphilic biodegradable copolymers:surface morphology control and biomedical application as scaffolds for cell growth[J]. ACS Appl.Mater.Interfaces, 2011,3:2487-2495. doi: 10.1021/am200371c
31. [31]
  Wu X.H., Wang S.F. Regulating MC3T3-E1 cells on deformable poly (e-caprolactone)honeycomb films prepared using a surfactant-free breath figure method in a water-miscible solvent[J]. ACS Appl.Mater.Interfaces, 2012,4:4966-4975. doi: 10.1021/am301334s
32. [32]
  Yap F.L., Zhang Y. Assembly of polystyrene microspheres and its application in cell micropatterning[J]. Biomaterials, 2007,28:2328-2338. doi: 10.1016/j.biomaterials.2007.01.034
33. [33]
  M.Hernández-Guerrero , Stenzel M.H. Honeycomb structured polymer films via breath figures[J]. Polym.Chem., 2012,3:563-577. doi: 10.1039/C1PY00219H
34. [34]
  Chen S.S., Lu X.M., Zhu D.D., Lu Q.H. Targeted grafting of thermoresponsive polymers from a penetrative honeycomb structure for cell sheet engineering[J]. Soft Matter, 2015,11:7420-7427. doi: 10.1039/C5SM01769F
35. [35]
  Chen S.S., Lu X.M., Hu Y., Lu Q.H. Biomimetic honeycomb-patterned surface as the tunable cell adhesion scaffold[J]. Biomater.Sci., 2015,3:85-93. doi: 10.1039/C4BM00233D
36. [36]
  Yin Y.D., Alivisatos A.P. Colloidal nanocrystal synthesis and the organic-inorganic interface[J]. Nature, 2005,437:664-670. doi: 10.1038/nature04165
37. [37]
  Kawano T., Sato M., Yabu H., Shimomura M. Honeycomb-shaped surface topography induces differentiation of human mesenchymal stem cells (hMSCs):uniform porous polymer scaffolds prepared by the breath figure technique[J]. Biomater.Sci., 2014,2:52-56. doi: 10.1039/C3BM60195A
38. [38]
  Biazar E., Khorasani M.T., Joupari M.D. Cell adhesion and surface properties of polystyrene surfaces grafted with poly(N-isopropylacrylamide)[J]. Chin.J.Polym. Sci., 2013,31:1509-1518. doi: 10.1007/s10118-013-1335-3
39. [39]
  The software was provided on the website: http://cn.mathworks.com/index.html?s_tid=gn_logo.
40. [40]
  Yao X., Peng R., Ding J.D. Cell-material interactions revealed via material techniques of surface patterning[J]. Adv.Mater., 2013,25:5257-5286. doi: 10.1002/adma.201301762
41. [41]
  Jeon H., C.G.Simon Jr., Kim G. A mini-review:cell response to microscale, nanoscale, and hierarchical patterning of surface structure[J]. J.Biomed.Mater. Res.Part B Appl.Biomater., 2014,102:1580-1594.
42. [42]
  Mosser D.M., Edwards J.P. Exploring the full spectrum of macrophage activation[J]. Nat.Rev.Immunol., 2008,8:958-969. doi: 10.1038/nri2448
43. [43]
  Dalby M.J., Gadegaard N., Oreffo R.O.C. Harnessing nanotopography and integrin-matrix interactions to influence stem cell fate[J]. Nat.Mater., 2014,13:558-569. doi: 10.1038/nmat3980
44. [44]
  Juan S.H., Tur J.M.M. Tensegrity frameworks:static analysis review[J]. Mech. Mach.Theory, 2008,43:859-881. doi: 10.1016/j.mechmachtheory.2007.06.010
45. [45]
  Zhang G.H., Hou R.X., Zhan D.X.. Fabrication of hollow porous PLGA microspheres for controlled protein release and promotion of cell compatibility[J]. Chin.Chem.Lett., 2013,24:710-714. doi: 10.1016/j.cclet.2013.05.011
46. [46]
  Heng L.P., Meng X.F., Wang B., Jiang L. Bioinspired design of honeycomb structure interfaces with controllable water adhesion[J]. Langmuir, 2013,29:9491-9498. doi: 10.1021/la401991n
47. [47]
  Dembo M., Wang Y.L. Stresses at the cell-to-substrate interface during locomotion of fibroblasts[J]. Biophys.J., 1999,76:2307-2316. doi: 10.1016/S0006-3495(99)77386-8
48. [48]
  Ingber D.E. Cellular tensegrity:defining new rules of biological design that govern the cytoskeleton[J]. J.Cell Sci., 1993,104:613-627.
49. [49]
  Ingber D.E., Tensegrity I. Cell structure and hierarchical systems biology[J]. J.Cell Sci., 2003,116:1157-1173. doi: 10.1242/jcs.00359
50. [50]
  Ingber D.E., Tensegrity I.I. How structural networks influence cellular information processing networks[J]. J.Cell Sci., 2003,116:1397-1408. doi: 10.1242/jcs.00360
51. [51]
  Ingber D.E. Tensegrity:the architectural basis of cellular mechanotransduction[J]. Annu.Rev.Physiol., 1997,59:575-599. doi: 10.1146/annurev.physiol.59.1.575
52. [52]
  Crawford-Young S.J.. Effects of microgravity on cell cytoskeleton and embryogenesis[J]. Int. J. Dev.Biol., 2006,50:183-191. doi: 10.1387/ijdb.052077sc
53. [53]
  Cogoli A., Tschopp A., Fuchs-Bislin P.. Cell sensitivity to gravity[J]. Science, 1984,225:228-230. doi: 10.1126/science.6729481
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