Citation: Song Ting-Ting, Wang Wei, Meng Li-Li, Liu Yan, Jia Xiao-Bo, Mao Xun. Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device[J]. Chinese Chemical Letters, ;2017, 28(2): 226-230. doi: 10.1016/j.cclet.2016.07.021 shu

Electrochemical detection of human ferritin based on gold nanorod reporter probe and cotton thread immunoassay device

Song Ting-Ting ^a,1 ,
Wang Wei ^b,1 ,
Meng Li-Li ^a ,
Liu Yan ^a ,
Jia Xiao-Bo ^a ,
Mao Xun ^a,,

a.
Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710127, China
b.
Center of Analysis, Guangdong Medical College, Dongguan 523808, China

Corresponding author: Mao Xun, xunmao@nwu.edu.cn

Received Date: 7 June 2016
Revised Date: 24 June 2016
Accepted Date: 5 July 2016
Available Online: 21 February 2016

Figures(6)

In this study, a natural cotton thread immunoassay device combined with gold nanorod (GNR) reporter probe is developed for the rapid, sensitive and quantitative electrochemical determination of human ferritin, a lung cancer related biomarker. Human ferritin as an analyte and a pair of monoclonal antibodies are used to demonstrate the proof-of-concept on the cotton thread immunoassay device. An enhancement of the sensitivity is achieved by using gold nanorod as an electroactive report probe compared with a traditional gold nanoparticle (GNP) report probe. The device was capable of measuring 1.58 ng/mL ferritin in 30 min by anodic stripping voltammetry (ASV) testing, which meet the requirement for clinical diagnosis.
- Cotton thread immunoassay device,
- Gold nanorod reporter probe,
- Point-of-care diagnosis,
- Protein detection,
- Electrochemical immunoassay
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