Citation: Shin-ichi Kawano, Hong-Yuan Hao, Yuki Hashi, Jin-Ming Lin. Analysis of chloramphenicol in honey by on-line pretreatment liquid chromatography-tandem mass spectrometry[J]. Chinese Chemical Letters, ;2015, 26(1): 36-38. doi: 10.1016/j.cclet.2014.10.026 shu

Analysis of chloramphenicol in honey by on-line pretreatment liquid chromatography-tandem mass spectrometry

1.
a Department of Chemistry, Beijing Key Laboratory of Microanalytical Methods and Instrumentation, Tsinghua University, Beijing 100084, China;
b Shimadzu Global COE for Application & Technical Development, Shimadzu (China) Co.
b Shimadzu Global COE for Application & Technical Development, Shimadzu (China) Co., Ltd., Shanghai 200052, China

Corresponding author: Jin-Ming Lin,
Received Date: 14 July 2014
Available Online: 15 October 2014
Fund Project: This work was supported by the National Natural Science Foundation of China (No. 21227006) (No. 21227006)the Research Fund for the Doctoral Program of Higher Education (No. 20110002110052). (No. 20110002110052)

Chloramphenicol is an antibiotic and one of the potential contaminants in honey. Solid-phase extraction is the key pretreatment procedure for analysis of chloramphenicol in honey. In this work, an on-line pretreatment liquid chromatography-tandem mass spectrometer system for sensitive, reliable and higher throughput analysis was developed. With the methylcellulose-immobilized reversed-phase column, sugars in a honey sample were efficiently removed in 1 min. As a result, the limit of quantitation of chloramphenicol was 20 pg/mL (0.2 mg/kg honey).
- On-line pretreatment,
- Liquid chromatography-mass spectrometry,
- Chloramphenicol,
- Honey
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沈阳化工大学材料科学与工程学院沈阳 110142