Citation: Jian-Xiang Huang, Da-Hai Lu, Kai Wan, Fu-Hua Wang. Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry[J]. Chinese Chemical Letters, ;2014, 25(4): 635-639. doi: 10.1016/j.cclet.2014.01.036 shu

Low temperature purification method for the determination of abamectin and ivermectin in edible oils by liquid chromatography-tandem mass spectrometry

1.
Public Monitoring Center for Agro-Product, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

Corresponding author: Fu-Hua Wang,
Received Date: 11 November 2013
Available Online: 13 January 2014
Fund Project: This research was financially supported by National Natural Science Foundation of China (No. 21305019) (No. 21305019) Special Fund for Agro-Scientific Research in the Public Interest (No. 201303088) (No. 201303088)President Fund of Guangdong Academy of Agricultural Sciences (No. 201218). (No. 201218)

In this study, a method based on low temperature purification (LTP) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of abamectin (ABA) and ivermectin (IVR) in edible oils. ABA and IVR were extracted using conventional liquid-liquid extraction followed by purification via precipitation of interfering fatty components at low temperature without an additional cleanup step. LTP is simple, easy to use, labour-saving and cost effective, and requires reduced amounts of organic solvent. The linear ranges of ABA and IVR were 5- 1000 μg/L using matrix-matched standards. Limits of detection (LOD) and limits of quantification (LOQ) were in the range of 0.1-0.4 μg/kg and 0.3-1.3 μg/kg, respectively. The LOQs were below the strictest maximum residue limits established by Codex Alimentarius Commission. Recoveries at three spiked levels of 10, 20 and 100 μg/kg in peanut oil, corn oil, olive oil, soybean oil and lard ranged from 71.1% to 119.3% with relative standard deviations of 3.2%-10.3%, which were in agreement with those obtained by the solid phase extraction method. The proposed method was utilized in the analysis of 10 edible oil samples from localmarket and neither ABA nor IVR was detected. As far as we know, this is the first time that LTP is applied to the determination of avermectins in edible oils.
- Low temperature purification,
- Abamectin,
- Ivermectin,
- Edible oil,
- Liquid chromatography-tandem mass,
- spectrometry
1. [1]
  [1] A.I. Valenzuela, M.J. Redondo, Y. Pico, G. Font, Determination of abamectin in citrus fruits by liquid chromatography-electrospray ionization mass spectrometry, J. Chromatogr. A 871 (2000) 57-65.
2. [2]
  [2] L. Howells, M.J. Sauer, Multi-residue analysis of avermectins and moxidectin by ion-trap LC-MSn, Analyst 126 (2001) 155-160.
3. [3]
  [3] Y. Zhang, C.M. Jiang, H. Chen, et al., Simultaneous detection of abamectin and ivermectin residue in eel with liquid chromatography-tandem mass spectrometry, Chin. J. Anal. Lab. 27 (2008) 104-107.
4. [4]
  [4] H.M. He, H. Zhao, C.R. Zhang, et al., Determination of abamectin residues in grain by ultra performance liquid chromatography-tandem mass spectrometry, Chin. J. Anal. Chem. 41 (2013) 1627-1632.
5. [5]
  [5] Determination of ivermectin, abamectin, doramectin and eprinomectin residues in fugu, eel and baked eel—LC-MS/MS method, National Standards of the Peoples Republic of China, GB/T 22953-2008.
6. [6]
  [6] Determination of ivermectin, abamectin, doramectin and eprinomectin residues in milk and milk powder—LC-MS/MS method, National Standards of the Peoples Republic of China, GB/T 22968-2008.
7. [7]
  [7] Maximum residue limits for pesticides in food, National Standards of the Peoples Republic of China, GB 2763-2012.
8. [8]
  [8] Report of the Codex Committee on Pesticide Residues, 24th session of the Codex Alimentarius Commission, 2001.
9. [9]
  [9] Report of the Codex Committee on Residues of Veterinary Drugs in Foods, 26th session of the Codex Alimentarius Commission, 2003.
10. [10]
  [10] G.K. Li, Y.L. Hu, G.H. Ruan, et al., Instruments and Devices of Sample Preparation, Chemical Industry Press, Beijing, 2007.
11. [11]
  [11] M.J. Hengel, M. Miller, Analysis of pesticides in dried hops by liquid chromatography- tandem mass spectrometry, J. Agric. Food Chem. 56 (2008) 6851-6856.
12. [12]
  [12] J.H. Borges, L.M. Ravelo-Pérez, E.M. Hernández-Suárez, et al., Determination of abamectin residues in avocados by microwave-assisted extraction and HPLC with fluorescence detection, Chromatogrphia 67 (2008) 69-75.
13. [13]
  [13] K.A. Krogh, E. Björklund, D. Loeffler, et al., Development of an analytical method to determine avermectins in water, sediments and soils using liquid chromatography- tandem mass spectrometry, J. Chromatogr. A 1211 (2008) 60-69.
14. [14]
  [14] H.M. He, H. Zhao, C.R. Zhang, et al., Determination of abamectin residue in paddy rice by ultra performance liquid chromatography-tandem mass spectrometry, Chin. J. Anal. Chem. 40 (2012) 140-144.
15. [15]
  [15] J. Hernández-Borges, L.M. Ravelo-Pérez, E.M. Hernández-Suárez, et al., Analysis of abamectin residues in avocados by high-performance liquid chromatography with fluorescence detection, J. Chromatogr. A 1165 (2007) 52-57.
16. [16]
  [16] M.L.G. Pérez, R. Romero-González, J.L.M. Vidal, et al., Analysis of veterinary drug residues in cheese by ultra-high-performance LC coupled to triple quadrupole MS/MS, J. Sep. Sci. 36 (2013) 1223-1230.
17. [17]
  [17] A. Giordano, M. Fernández-Franzón, M.J. Ruiz, et al., Pesticide residue determination in surface waters by stir bar sorptive extraction and liquid chromatography/ tandem mass spectrometry, Anal. Bioanal. Chem. 393 (2009) 1733-1743.
18. [18]
  [18] A.M. Filho, F.N.D. Santos, P.A.D.P. Pereira, Multi-residue analysis of pesticide residues in mangoes using solid-phase microextraction coupled to liquid chromatography and UV-vis detection, J. Sep. Sci. 34 (2011) 2960-2966.
19. [19]
  [19] X. Xia, Z.M. Xiao, Q.S. Huang, Simultaneous determination of avermectin and milbemycin residues in bovine tissue by pressurized solvent extraction and LC with fluorescence detection, Chromatographia 72 (2010) 1089-1095.
20. [20]
  [20] R.A. Lorenzo, S. Pais, I. Racamonde, et al., Pesticides in seaweed: optimization of pressurized liquid extraction and in-cell clean-up and analysis by liquid chromatography- mass spectrometry, Anal. Bioanal. Chem. 404 (2012) 173-181.
21. [21]
  [21] J.H. Park, J.H. Choi, A.M.A. EI-Aty, et al., Development of an extraction method for the determination of avermectins in soil using supercritical CO2 modified with ethanol and liquid chromatography-tandem mass spectrometry, J. Sep. Sci. 36 (2013) 148-155.
22. [22]
  [22] X.L. Hou, X.W. Li, S.Y. Ding, et al., Simultaneous analysis of avermectins in bovine tissues by LC-MS-MS with immunoaffinity chromatography cleanup, Chromatographia 63 (2006) 543-550.
23. [23]
  [23] C. Lentza-Rizos, E.J. Avramides, F. Cherasco, Low-temperature clean-up method for the determination of organophosphorus insecticides in olive oil, J. Chromatogr. A 912 (2001) 135-142.
24. [24]
  [24] S.M. Goulart, M.E.L.R.D. Queiroz, A.A. Neves, J. Dequeiroz, Low-temperature cleanup method for the determination of pyrethroids in milk using gas chromatography with electron capture detection, Talanta 75 (2008) 1320-1323.
25. [25]
  [25] C. Lentza-Rizos, E.J. Avramides, E. Visi, Determination of residues of endosulfan and five pyrethroid insecticides in virgin olive oil using gas chromatography with electron-capture detection, J. Chromatogr. A 921 (2001) 297-304.
26. [26]
  [26] L. Li, H.Y. Zhang, C.P. Pan, et al., Multiresidue analytical method of pesticides in peanut oil using low-temperature cleanup and dispersive solid phase extraction by GC-MS, J. Sep. Sci. 30 (2007) 2097-2104.
27. [27]
  [27] T.D. Nguyen, M.H. Lee, G.H. Lee, Rapid determination of 95 pesticides in soybean oil using liquid-liquid extraction followed by centrifugation, freezing and dispersive solid phase extraction as cleanup steps and gas chromatography with mass spectrometric detection, Microchem. J. 95 (2010) 113-119.
28. [28]
  [28] P.D. Andrade, J.L.G.D. Silva, E.D. Caldas, Simultaneous analysis of aflatoxins B1, B2, G1, G2, M1 and ochratoxin A in breast milk by high-performance liquid chromatography/ fluorescence after liquid-liquid extraction with low temperature purification (LLE-LTP), J. Chromatogr. A 1304 (2013) 61-68.
29. [29]
  [29] G. Rübensam, F. Barreto, R.B. Hoff, T.M. Pizzolato, A liquid-liquid extraction procedure followed by a low temperature purification step for the analysis of macrocyclic lactones in milk by liquid chromatography-tandem mass spectrometry and fluorescence detection, Anal. Chim. Acta 705 (2011) 24-29.
30. [30]
  [30] G.P.D. Pinho, A.A. Neves, M.E.L.R.D. Queiroz, F.O. Silvoôrio, Optimization of the liquid-liquid extraction method and low temperature purification (LLE-LTP) for pesticide residue analysis in honey samples by gas chromatography, Food Control 21 (2010) 1307-1311.
31. [31]
  [31] G.P.D. Pinho, A.A. Neves, M.E.L.R.D. Queiroz, F.O. Silvoôrio, Pesticide determination in tomatoes by solid-liquid extraction with purification at low temperature and gas chromatography, Food Chem. 121 (2010) 251-256.
32. [32]
  [32] G. Rübensam, F. Barreto, R.B. Hoff, et al., Determination of avermectin and milbemycin residues in bovine muscle by liquid chromatography-tandem mass spectrometry and fluorescence detection using solvent extraction and low temperature cleanup, Food Control 29 (2013) 55-60.
33. [33]
  [33] E.J. Magalhaães, M.E.L.R.D. Queiroz, M.L.D.O. Penido, et al., Determination of cocaine in postmortem human liver exposed to overdose. Application of an innovative and efficient extraction/clean up procedure and gas chromatography- mass spectrometry analysis, J. Chromatogr. A 1309 (2013) 15-21.
34. [34]
  [34] S.K. Cho, A.M.A. El-Aty, K.H. Park, et al., Simple multiresidue extraction method for the determination of fungicides and plant growth regulator in bean sprouts using low temperature partitioning and tandem mass spectrometry, Food Chem. 136 (2013) 1414-1420.
35. [35]
  [35] F.A. Esteve-Turrillas, A. Pastor, M.D.L. Guardia, Determination of pyrethroid insecticide residues in vegetable oils by using combined solid-phases extraction and tandem mass spectrometry detection, Anal. Chim. Acta 553 (2005) 50-57.
36. [36]
  [36] E.G. Amvrazi, T.A. Albanis, Multiresidue method for determination of 35 pesticides in virgin olive oil by using liquid-liquid extraction techniques coupled with solid-phase extraction clean up and gas chromatography with nitrogen phosphorus detection and electron capture detection, J. Agric. Food Chem. 54 (2006) 9642-9651.
37. [37]
  [37] S. Jiang, Y.S. Li, B. Sun, Determination of trace level of perchlorate in Antarctic snow and ice by ion chromatography coupled with tandem mass spectrometry using an automated sample on-line preconcentration method, Chin. Chem. Lett. 24 (2013) 311-314.
38. [38]
  [38] L.N. Chen, F.R. Song, Z. Zheng, et al., Studies on the determination method of pesticide multi-residues in ginseng by ultra performance liquid chromatography tandem mass spectrometry, Acta Chim. Sin. 70 (2012) 843-851.
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