Citation: Yang Ping Wen, Li Min Lu, Dong Li, Ming Liu, Hao Hua He, Jing Kun Xu. Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) matrices for agricultural application in crops[J]. Chinese Chemical Letters, ;2012, 23(2): 221-224. doi: 10.1016/j.cclet.2011.11.010 shu

Ascorbate oxidase electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) matrices for agricultural application in crops

Yang Ping Wen ^a,b, ,
Li Min Lu ^a ,
Dong Li ^a,b ,
Ming Liu ^a,b ,
Hao Hua He ^a ,
Jing Kun Xu ^b

a Key Lab of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, China;
b Jiangxi Key Lab of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang 330013, China

Received Date: 19 July 2011

Fund Project: This research was supported by NSFC (Nos. 50963002 and 51073074), Key Projects in the National Science & Technology Pillar Program in the Eleventh Five-year Plan Period of China (Nos. 2006BAD02A04 and 2006BAD01A01), Jiangxi Provincial Department of Education (Nos. GJJ11590 and GJJ10678).

The vitamin C (VC) in crops was successfully determined using ascorbate oxidase (AO) electrochemical biosensor based on the biocompatible poly(3, 4-ethylenedioxythiophene) (PEDOT) matrices, which was easily prepared by one-step electrodeposition technique in ionic liquid microemulsions. The fabricated biosensor displayed excellent bioelectrocatalytic performance to the oxidation of VC, wide linear range, low detection limit, fast response time, good operational and storage stability, the good results of the determination of VC in vegetable crops indicated that the fabricated biosensor will be a good candidate for the physiological and biochemical studies of crops in near future.
- Biosensor,
- Agricultural application,
- Ionic liquid microemulsions,
- Ascorbate oxidase,
- Poly(3, 4-ethyl-enedioxythiophene),
- Vitamin C
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