Citation: Chun Cui Huang, Hui Bo Shao. Enhanced flow injection analysis for measurements of S-nitrosothiols species in biological samples using highly selective amperometric nitric oxide sensor[J]. Chinese Chemical Letters, ;2012, 23(2): 229-232. doi: 10.1016/j.cclet.2011.11.003 shu

Enhanced flow injection analysis for measurements of S-nitrosothiols species in biological samples using highly selective amperometric nitric oxide sensor

Key Laboratory of Cluster Science, Ministry of Education of China, School of Chemistry, Beijing Institute of Technology, Beijing 100081, China

Received Date: 23 August 2011

Fund Project: This work was partially completed in the University of Michigan, and helpful instructions and paper revision from Prof. Mark E. Meyerhoff are gratefully acknowledged. We are indebted to the Project of Base for Introducing Talents of Discipline to Universities (111 project, No. B07012). National Scholarship Fund of the China Scholarship Council for supporting visiting students in foreign universities is greatly acknowledged.

A highly selective nitric oxide (NO) sensor is fabricated and applied to devise an enhanced flow injection analysis (FIA) system for S-nitrosothiols (RSNOs) measurement in biological samples. The NO sensor is prepared using a polytetrafluoroethylene (PTFE) gas-permeable membrane loaded with Teflon AF^® solution, a copolymer of tetrafluoroethylene and 2, 2-bis(trifluoroethylene)-4, 5-difluoro-1, 3-dioxole, to improve selectivity. This method is much simpler and possesses good performance over a wide range of RSNOs concentrations. Standard deviation for three parallel measurements of blood plasma is 4.0%. The use of the gas sensing configuration as the detector enhances selectivity of the FIA measurement vs. using less selective electrochemical detectors that do not use PTFE/Teflon type outer membranes.
- Enhanced FIA method,
- Plasma,
- RSNOs,
- Highly selective NO sensor
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