Citation: Jie Hua, Ke Long Huang. Optimization by orthogonal array design of ion-pair HPLC separation of the enzymatic hydrolysis products of yeast RNA[J]. Chinese Chemical Letters, ;2010, 21(12): 1453-1456. doi: 10.1016/j.cclet.2010.07.002 shu

Optimization by orthogonal array design of ion-pair HPLC separation of the enzymatic hydrolysis products of yeast RNA

Jie Hua ^a,b, ,
Ke Long Huang ^a

a School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China;
b School of Chemistry and Chemical Engineering, Hunan Institute of Science and Technology, Yueyang 414000, China

Received Date: 23 March 2010

The separation of enzymatic hydrolysis products of yeast RNA by ion-pair HPLC was studied. A modified chromatographic response function (MCRF) was proposed to appraise the effectiveness of chromatographic separation. This function takes the number of peaks, resolution and the retention time of the last peak into consideration. It shows advantages for optimization of HPLC separation of complex mixtures. An orthogonal array design was used to separate the hydrolysate of yeast RNA and the optimal chromatographic conditions were obtained.
- Chromatographic response function,
- Orthogonal array design,
- 5'-Phosphodiesterase,
- Barley roots,
- Nucleotides
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