Citation: Ying Xia Wang, Yong Nian Ni, Serge Kokot. Investigation of the interaction of carbofuran and DNA by voltammetry and fluorescence with the aid of chemometrics[J]. Chinese Chemical Letters, ;2010, 21(8): 963-967. doi: 10.1016/j.cclet.2010.03.015 shu

Investigation of the interaction of carbofuran and DNA by voltammetry and fluorescence with the aid of chemometrics

a State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;
b Department of Chemistry, Nanchang University, Nanchang 330031, China;
c School of Physical and Chemical Sciences, Queensland University of Technology, Brisbane, Qld 4001, Australia

Received Date: 18 December 2009

Fund Project: The authors gratefully acknowledge the financial support by the State Key Laboratory of Food Science and Technology of Nanchang University (Nos. SKLF-MB-200807 and SKLF-TS-200819).

The interactions of carbofuran and DNA were studied using voltammetry and fluorescence spectroscopy.The formation of carbofuran-DNA makes the current peak of DNA decreased by voltammetry method.The binding number (n) and constant (K_a) for complex carbofuran-DNA were calculated to be 1.06±0.04 and 0.11±0.03 mol^-1 L,respectively by fluorescence measurement.Chemometrics approach,such as singular valueal species in the drug-DNA binding process.And the pure spectra and concentration profiles in the kinetic system were clearly deduced by multivariate curve resolution alternating least squares (MCR-ALS) with the initial estimates by evolving factor analysis (EFA).
- Carbofuran,
- DNA,
- Voltammetry,
- Chemometrics
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