Citation: Dong Ju, Sheng Mei Song, Yan Bo Wu, Shao Min Shuang, Chuan Dong. Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin[J]. Chinese Chemical Letters, ;2010, 21(4): 480-483. doi: 10.1016/j.cclet.2009.11.042 shu

Interaction between a novel intramolecular charge transfer fluorescent probe PFEP and human serum albumin

Research Center of Environmental Science and Engineering, College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China

Received Date: 17 August 2009

Fund Project: This work was supported by the National Natural Science Foundation of China (No. 20875059).

The interaction mechanism between human serum albumin(HSA)and 1-phenyl-3(fluorenone-2-yl)-5-(9-ethylcarbazole-3-yl)-2-pyrazoline(PFEP)was investigated by fluorescence and absorption titration techniques in combination with molecular modeling method.Stern-Volmer plots at different temperatures proved that PFEP could quench the intrinsic fluorescence of HSA attributed to a static quenching procedure.The association constants were calculated in the range of 1×10⁵-8×10⁵ mol^-1 at different pH conditions,and the stoicbiometric ratio of binding was 1:1 between PEEP and HSA.Molecular modeling study showed that the distance between indole moiety of the Trp214 residue and the carbazole group at the terminal of PFEP was 4.45 Å in the optimal model.
- Fluorescent probe,
- Human serum albumin,
- ICT
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