Citation: G. Rezaei Behbehani, A. A. Saboury, M. Mohebbian, S. Ghammamy. Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease[J]. Chinese Chemical Letters, ;2010, 21(4): 457-460. doi: 10.1016/j.cclet.2009.10.021 shu

Application of a simple calorimetric data analysis on the binding study of cyanide ions by Jack bean urease

a Chemistry Department, Imam Khomeini International University, Qazvin, Iran;
b Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran;
c Chemistry Department, Payame Noor University(PNU), Abhar, Iran

Received Date: 14 July 2009

Fund Project: Financial support from the Universities of Imam Khomeini International University, Tehran and Payame Noor (Abhar) are gratefully acknowledged.

Cyanide ion was studied as an effector of Jack bean urease(JBU)at 300 K in 30 mmol/L Tris buffer,pH 7 by isothermal titration calorimetry(ITC).The simple novel model was used for CN^-+JBU interaction over the whole range of CN^-concentrations.The binding parameters recovered from the simple novel model were attributed to the cyanide ion interaction.It was found that cyanide ion acted as a noncooperative inhibitor of JBU,and there is a set of 12 identical and independent binding sites for CN^-ions.The dissociation equilibrium constant is 750 μmol/L.The molar enthalpy of binding is △H=-13.6 kJ mol^-1.The technique used provided an accurate and quick assessment of the effectiveness of the compounds to inhibit Jack bean urease.
- Isothermal titration calorimetry,
- Cyanide ion,
- Jack bean ttrease,
- Binding parameter
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