Citation: Chuan Yin Liu, Ji Ming Hu. Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized on L-glutathione self-assembled monolayers[J]. Chinese Chemical Letters, ;2008, 19(8): 955-958. doi: 10.1016/j.cclet.2008.04.023 shu

Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized on L-glutathione self-assembled monolayers

Chuan Yin Liu ^a,b, ,
Ji Ming Hu ^b

a Department of Chemistry, Yunyang Teachers College, Danjiangkou 442700, China;
b School of Chemistry & Molecular Sciences, Wuhan University, Wuhan 430072, China

Received Date: 14 January 2008

A novel hydrogen peroxide biosensor has been fabricated based on covalently linked horseradish peroxidase (HRP) onto Lglutathione self-assembled monolayers (SAMs).The SAMs-based electrode was characterized by electrochemical methods, and direct electrochemistry of HRP can be achieved with formal potential of-0.242 V (vs.saturated Ag/AgCl) in pH7 phosphate buffer solution (PBS), the redox peak current is linear to scan rate and rate constant can be calculated to be 0.042 s^-1.The HRP-SAMs-based biosensors show its better electrocatalysis to hydrogen peroxide in the concentration range of 1×10^-6 mol/L to 1.2×10^-3 mol/L with a detection limit of 4×10^-7 mol/L.The apparent Michealis-Menten constant is 3.12 mmol/L.The biosensor can effectively eliminate the interferences of dopamine, ascorbic acid, uric acid, catechol and p-acetaminophen.
- Direct electrochemistry,
- Horseradish peroxidase,
- Biosensor,
- Self-assembled monolayen, Hydrogen peroxide
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