Citation: Yu Dong Shen, Yu Wang, Shi Wei Zhang, Zhi Li Xiao, Yuan Ming Sun, Xian Zhang Bu, Lian Quan Gu. Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone[J]. Chinese Chemical Letters, ;2007, 18(12): 1490-1492. doi: 10.1016/j.cclet.2007.10.030 shu

Design and efficient synthesis of novel haptens and complete antigens for the AOZ,a toxic metabolite of furazolidone

a Food Quality and Safety Key Laboratory of Department of Education of Guangdong Province/Institute of Food Quality and Safety, South China Agricultural University, Guangzhou 510642, China;
b School of Pharmaceutical Sciences, Sun Yat-Sen University, Guangzhou 510080, China

Received Date: 27 August 2007

Fund Project: The work was supported by the Natural Science Foundation of Guangdong Province (Nos.06300421 and 06300423), the Headmaster's Science Fund of High Schools (No.5100-k06133) and Public Bidding Items Fund for a Great Breakthrough in Key Fields (No.2006A25005002).

A good strategy was brought forward for designing efficient haptens and complete antigens for 3-amino-2-oxazolidinone (AOZ). Haptens designed newly were achieved facilely in good yield by using LiCl-N (Et)₃ as new catalysis system, the structure of which was elucidated by spectroscopy analysis, such as NMR and MS. Novel antigens for AOZ were prepared successfully by convenient ester method. The ratios of haptens 3 and 4 to carrier proteins were proven respectively as 41:1 (5a), 39:1 (6a), 11:1 (5b) and 9:1 (6b) by trinitrobenzene sulfonic acid (TNBS) method. The results of indirect competitive ELISA (ic-ELISA) of antiserums indicated that the haptens with a short unsaturated side chain can evoke specific immune response effectively.
- Furazolidone,
- AOZ,
- Antigen,
- Hapten,
- Immunoassay
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