Citation: Kai An Yao, Na Wang, Jing Yue Zhuang, Zheng Biao Yang, Hai Yan Ni, Quan Xu, Cheng Sun, Shu Ping Bi. Differential pulse voltammetric studies on the effects of Al(Ⅲ) on the lactate dehydrogenase activity[J]. Chinese Chemical Letters, ;2007, 18(7): 857-860. doi: 10.1016/j.cclet.2007.05.011 shu

Differential pulse voltammetric studies on the effects of Al(Ⅲ) on the lactate dehydrogenase activity

State Key Laboratory of Pollution Control and Resource Reuse & Key Laboratory of MOE for Life Science, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China

Received Date: 4 January 2007

Fund Project: This project supported by the Natural Science Foundation of Jiangsu Province (BK 2005083), Grant of Analytical Measurements of Nanjing University, The National Science Foundation of China (No. 20575025) and Research Funding for the Doctoral Program of Higher Education (No. 20050284030).

In this paper, differential pulse voltammetry (DPV) was applied to study the effects of aluminum Al(Ⅲ) on the lactate dehydrogenase (LDH) activity. Michaelis-Menten constant (K_m^NADH) and maximum velocity (υ_max) in the enzyme promoting catalytic reaction of "pyruvate(Pyr)+NADH+H⁺ ??? lactate+NAD⁺" under different conditions by monitoring DPV reduction current of NAD⁺ were reported.
- Al(Ⅲ),
- DPV,
- Lactate dehydrogenase,
- Biomarker
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