Citation: Bin Guan, Dong Xing Yuan. Determination of neomycin in water samples by high performance anion chromatography with pulsed amperometric detection[J]. Chinese Chemical Letters, ;2007, 18(2): 201-204. doi: 10.1016/j.cclet.2006.12.022 shu

Determination of neomycin in water samples by high performance anion chromatography with pulsed amperometric detection

Bin Guan ,
Dong Xing Yuan

Environmental Science Research Center, Xiamen University, Xiamen 361005, China

Received Date: 25 September 2006

A simple, fast and reliable method, using high performance anion chromatography with pulsed amperometric detection, had been developed for the analysis of neomycin in water samples. The elution and separation were carried out with an isocratic mobile phase, containing 10 mmol/L NaOH. The influence of the concentration and pH of the mobile phase on the separation and detection was investigated. A quadruple-potential waveform used for the detection was optimized. The detection limit of neomycin was down to 0.027 μg/mL. The linearity of neomycin calibration curve ranged from 0.050 to 0.505 μg/mL with correlation coefficient of0.9997. R.S.D. (n=11) was 4.0%.
- Neomycin,
- High performance anion chromatography,
- Pulsed amperometric detection
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