Citation: De Bin Zhu, Da Xing, Ya Bing Tang. Highly sensitive ECL-PCR method for detection of K-ras point mutation[J]. Chinese Chemical Letters, ;2007, 18(2): 198-200. doi: 10.1016/j.cclet.2006.12.017 shu

Highly sensitive ECL-PCR method for detection of K-ras point mutation

MOE Key Laboratory of Laser Life Science and Institute of Laser Life Science, South China Normal University, Guangzhou 510635, China

Received Date: 7 July 2006

Fund Project: 60378043 30470494),and the Natural Science Foundation of Guangdong Province (Nos. 015012This research is supported by the National Natural Science Foundation of China (Nos. 30600128 2004B10401011). 04010394

A highly sensitive electrochemiluminescence-polymerase chain reaction (ECL-PCR) method for K-ras point mutation detection is developed. Briefly, K-ras oncogene was amplified by a Ru(bpy)₃²⁺ (TBR)-labeled forward and a biotin-labeled reverse primer,and followed by digestion with MvaI restriction enzyme, which only cut the wild-type amplicon containing its cutting site. The digested product was then adsorbed to the streptavidin-coated microbead through the biotin label and detected by ECL assay. The experiment results showed that the different genotypes can be clearly discriminated by ECL-PCR method. It is useful in point mutation detection, due to its sensitivity, safety, and simplicity.
- Electrochemiluminescence-polymerase chain reaction,
- K-ras oncogene,
- Point mutation detection
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