源自绿萝叶提取物的红光碳点制备和生物成像应用

引用本文: 邵仁义, 库拉姆·阿巴斯, 弗拉基米尔·尤里耶维奇·奥西波夫, 朱海梅, 李远, 乌萨马, 毕红. 源自绿萝叶提取物的红光碳点制备和生物成像应用[J]. 物理化学学报, 2026, 42(2): 100134. doi: 10.1016/j.actphy.2025.100134 shu

Citation: Renyi Shao, Khurram Abbas, Vladimir Yu. Osipov, Haimei Zhu, Yuan Li, Usama, Hong Bi. Red-emitting carbon dots prepared from Epipremnum Aureum leaves extract for biological imaging[J]. Acta Physico-Chimica Sinica, 2026, 42(2): 100134. doi: 10.1016/j.actphy.2025.100134 shu

源自绿萝叶提取物的红光碳点制备和生物成像应用

1.
安徽大学材料科学与工程学院, 安徽合肥 230601
2.
安徽大学化学化工学院, 安徽合肥 230601
3.
Ioffe Institute, St. Petersburg 194021, Russian Federation

通讯作者: 24743@ahu.edu.cn (乌萨马); Email: bihong@ahu.edu.cn (毕红)

摘要: 荧光碳点(CDs)已经广泛应用于生物体外/体内的荧光成像。然而，荧光CDs作为生物标记的特异性较差、而且在红光发射区域荧光量子产率(QY)较低，仍然是亟待解决的关键问题。本研究以天然植物绿萝叶片的乙醇提取液为前驱体，通过溶剂热“一锅”法合成了一种红色荧光碳点(命名为EA-CDs，λ_ex/λ_em = 400 nm/660 nm)。EA-CDs具有小尺寸(平均粒径3.9 nm)、高荧光QY (在乙醇中λ_em = 660 nm，QY = 15.4%)、低毒性(体外/体内)和良好的亲脂性(油水分离系数LogP > 0)，因此适用于体外/体内生物荧光成像和标记。实验结果表明该红光CDs不仅可以用于标记植物细胞膜，而且可以作为斑马鱼体内肠道荧光成像工具，有望成为一种通用型具有高荧光量子产率的生物膜红光染料。此外，本工作也为绿萝等观赏性植物的应用开拓了一种新思路。

关键词:

English

Red-emitting carbon dots prepared from Epipremnum Aureum leaves extract for biological imaging

Renyi Shao ^1, ,
Khurram Abbas ^2, ,
Vladimir Yu. Osipov ^3, ,
Haimei Zhu ^1, ,
Yuan Li ^1, ,
Usama ^{1,
,} ,
Hong Bi ^{1,
,}

1.
School of Materials Science and Engineering, Anhui University, Hefei 230601, Anhui Province, China
2.
School of Chemistry and Chemical Engineering, Anhui University, Hefei 230601, Anhui Province, China
3.
Ioffe Institute, St. Petersburg 194021, Russian Federation

Corresponding author: 24743@ahu.edu.cn (Usama); Email: bihong@ahu.edu.cn (Hong Bi)

Received Date: 06 June 2025
Accepted Date: 22 July 2025
Revised Date: 17 July 2025
Available Online: 15 February 2026

Abstract: Carbon dots (CDs) have been widely applied in fluorescence imaging both in vitro and in vivo. However, key challenges remain to be addressed, including the poor specificity of CDs as biological markers and their relatively low fluorescence quantum yield (QY) in the red emission region. In this study, we synthesized red fluorescent carbon dots (designated as EA-CDs, λ_ex/λ_em = 400 nm/660 nm) using a natural plant-derived precursor ethanol extract from Epipremnum aureum leaves via a one-pot solvothermal method. The EA-CDs exhibit a small particle size (average diameter: 3.9 nm), high fluorescence QY (15.4% in ethanol at λ_em = 660 nm), low toxicity (both in vitro and in vivo), and favorable lipophilicity (oil-water partition coefficient LogP > 0), making them suitable for biological fluorescence imaging and labeling applications. Experimental results indicate that these red-emitting CDs can not only effectively label plant cell membranes, but also serve as an intestinal fluorescence imaging probe in zebrafish models. This suggests their potential as a universal red-emissive bio-membrane dye with high QY. Furthermore, this work pioneers a novel application approach for ornamental plants like Epipremnum aureum.

Key words:

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通讯作者: 陈斌, bchen63@163.com

1.
沈阳化工大学材料科学与工程学院沈阳 110142

源自绿萝叶提取物的红光碳点制备和生物成像应用

通讯作者: 24743@ahu.edu.cn (乌萨马); Email: bihong@ahu.edu.cn (毕红)

English

Red-emitting carbon dots prepared from Epipremnum Aureum leaves extract for biological imaging

Corresponding author: 24743@ahu.edu.cn (Usama); Email: bihong@ahu.edu.cn (Hong Bi)

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通讯作者: 陈斌, bchen63@163.com

中国化学会秘书处

源自绿萝叶提取物的红光碳点制备和生物成像应用

通讯作者: 24743@ahu.edu.cn (乌萨马); Email: bihong@ahu.edu.cn (毕红)

English

Red-emitting carbon dots prepared from Epipremnum Aureum leaves extract for biological imaging

Corresponding author: 24743@ahu.edu.cn (Usama); Email: bihong@ahu.edu.cn (Hong Bi)

CCS Chemistry：嵌段共聚物自组装成 “三明治”二维有机材料，实现纳米级压力传感功能

CCS Chemistry：颜徐州、鲍哲南： 你的皮肤可能是一片SPMs

CCS Chemistry：工作高效不疲劳，只须让催化剂动起来

CCS Chemistry：静电组装导向聚合- 聚电解质纳米凝胶量化制备新策略

CCS Chemistry：金黄色葡萄球菌醛基脱氢酶是如何识别特异性底物的？

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通讯作者: 陈斌, bchen63@163.com

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