Citation: Xiaoxiao Guo, Pei Xu, Minhua Zong, Wenyong Lou. Purification and characterization of alkaline chitinase from Paenibacillus pasadenensis CS0611[J]. Chinese Journal of Catalysis, ;2017, 38(4): 665-672. doi: 10.1016/S1872-2067(17)62787-6 shu

Purification and characterization of alkaline chitinase from Paenibacillus pasadenensis CS0611

Xiaoxiao Guo ^a, ,
Pei Xu ^a ,
Minhua Zong ^b ,
Wenyong Lou ^a,b

a Laboratory of Applied Biocatalysis, School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China;
b State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou 510640, Guangdong, China

Received Date: 28 November 2016
Revised Date: 14 January 2017

Fund Project: This work was supported by the National Natural Science Foundation of China (21336002, 21376096, 21676104).

An extracellular chitinase produced by Paenibacillus pasadenensis CS0611 was purified by ammonium sulfate precipitation, HiTrap DEAE FF and HiLoad 26/600 Superdex 200pg column chromatography. The apparent molecular mass determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis was 69 kDa. The optimum pH and optimum temperature of the chitinase were 5.0 and 50℃, respectively. The enzyme showed high stability at alkaline pH values and temperatures below 40℃. Additionally, the metal ions Mn²⁺, Mg²⁺, and Co²⁺ inhibited activity of the chitinase. The chitinase was active on colloidal chitin with an apparent K_m of 4.41 mg/mL and V_max of 1.08 mg/min. Substrate spectrum analysis indicated that the chitinase reacted preferentially with the glucosidic bond between GlcNAc-GlcNAc. The enzymatic hydrolysate was analyzed by high-performance liquid chromatography and thin layer chromatography, and clearly showed that a subunit of (GlcNAc)₂ was the main hydrolysis product.
- Chitinase,
- Purification,
- Characterization,
- Paenibacillus pasadenensis CS
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