Citation: Ángela García Solaesa, María Teresa Sanz, Sagrario Beltrán, Rodrigo Melgosa. Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium[J]. Chinese Journal of Catalysis, ;2016, 37(4): 596-606. doi: 10.1016/S1872-2067(15)61040-3 shu

Kinetic study and kinetic parameters of lipase-catalyzed glycerolysis of sardine oil in a homogeneous medium

1.
Department of Biotechnology and Food Science (Chemical Engineering Section), University of Burgos, Burgos 09001, Spain

Corresponding author: María Teresa Sanz,
Received Date: 18 November 2015
Available Online: 4 January 2016

The production of polyunsaturated fatty acids (PUFAs) concentrates by enzymatic catalysis has gained interest due to their stereospecificity and the milder conditions employed compared to the use of inorganic catalysts. The enzymatic glycerolysis of sardine oil by Lipozyme® 435 to get PUFA concentrates in the forms of di- and monoacylglycerols (DAGs, MAGs) in an optimized amount of tert-butanol as the organic solvent was studied. First, mass transfer limitation of the reaction system was analyzed. The effects of different operating variables such as lipase loading, temperature and feed composition were investigated. A semi-empirical kinetic model based on the reversible elementary reactions of glycerolysis and hydrolysis of the glycerides was employed to correlate the experimental kinetic data. A molar ratio glycerol:oil of 3:1 was the optimum, which produced more than 84 wt% of MAG at 323 K. A comparison with other glycerolysis systems was performed using MAG yield, reaction rate and significance of kinetic parameters.
- Lipase,
- Glycerolysis,
- Tert-butanol,
- Mass transfer,
- Kinetic model
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