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Citation: GUO Su, LIAO Wei, WEI Fang, QIAN Min-Xie, ZHAO Xin-Sheng. Protein Biosensors Based on Scanning Potential Hairpin Denaturation and Fluorescence Resonance Energy Transfer[J]. Acta Physico-Chimica Sinica, ;2006, 22(08): 917-920. doi: 10.1016/S1872-1508(06)60040-5 shu

Protein Biosensors Based on Scanning Potential Hairpin Denaturation and Fluorescence Resonance Energy Transfer

1.
Beijing National Laboratory for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species,and Department of Chemical Biology, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, P. R.China

Received Date: 21 April 2006
Available Online: 7 July 2006

The ability of detecting multiple analytes simultaneously is an important advantage of protein sensors. But the denaturation of proteins on surfaces is still a challenge. In this paper, a protein biosensor providing high selectivity is introduced. This approach is based on DNA-Directed Immobilization (DDI) method, in which protein-DNA conjugates are immobilized on surface through DNA hybridization. Nonspecific binding is reduced by applying fluorescence resonance energy transfer (FRET) and scanning potential hairpin denaturation (SPHD) technologies. Excellent discrimination on different recognition patterns is obtained.
- Proteins;Biosensor;FRET;Potential control;SPHD;Microarray
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