Citation: Vadim V. Annenkov, Uma Maheswari Krishnan, Viktor A. Pal'shin, Stanislav N. Zelinskiy, Gayathri Kandasamy, Elena N. Danilovtseva. Bioinspired Water-soluble Polymers with Grafted Polyamine Chains: Synthesis and Complexation with Oligonucleotides[J]. Chinese Journal of Polymer Science, ;2018, 36(10): 1114-1122. doi: 10.1007/s10118-018-2133-8 shu

Bioinspired Water-soluble Polymers with Grafted Polyamine Chains: Synthesis and Complexation with Oligonucleotides

a.
Limnological Institute of the Siberian Branch of the Russian Academy of Sciences, 3, Ulan-Batorskaya St., P.O. Box 278, Irkutsk, 664033, Russia
b.
Centre for Nanotechnology & Advanced Biomaterials (CeNTAB), School of Chemical and Biotechnology, SASTRA University, Thanjavur – 613401, Tamil Nadu, India

Corresponding author: Vadim V. Annenkov, annenkov@lin.irk.ru
Received Date: 18 January 2018
Revised Date: 25 February 2018
Accepted Date: 3 March 2018
Available Online: 19 April 2018

The siliceous frustules of diatom algae contain complex proteins known as silaffins, which consist of a peptide chain with grafted polyamine chains. These polyamines contain twenty or more nitrogen atoms with trimethylene groups between the nitrogens. We synthesized a set of polymers containing grafted long-chain polyamine fragments by using acryloyl chloride (ACh) polymers and activated acrylic acid copolymers as the starting materials. The new polymers contained 0.05 mol%−3.2 mol% of polyamine chains, which corresponded to 0.06−3.56 mmol·g⁻¹ amine groups. The new amine-containing polymers formed complexes with short (19-21-mer) deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) strands, and these complexes penetrated into model yeast cells and A549 lung cancer cell. This study demonstrates the potential of these species based on long-chain polyamines to serve as novel gene delivery systems.
- Polyamine,
- Polymeric amine,
- Gene delivery,
- Acryloyl chloride
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