Citation: Cong-heng Chen, Juan Zhao, Zhou Yang, Qing Sun, Ping Zhou. Nanofibers of Silk Fibroin Controlled by the Crystallization of Polyethylene Glycol in Frozen Solution[J]. Chinese Journal of Polymer Science, ;2017, 35(11): 1373-1380. doi: 10.1007/s10118-017-1974-x shu

Nanofibers of Silk Fibroin Controlled by the Crystallization of Polyethylene Glycol in Frozen Solution

State Key Laboratory of Molecular Engineering of Polymers, Department of Macromolecular Science, Fudan University, Shanghai 200433, China

Corresponding author: Ping Zhou, pingzhou@fudan.edu.cn
Received Date: 15 March 2017
Revised Date: 28 April 2017
Accepted Date: 8 May 2017

Fund Project: the National Natural Science Foundation of China 21374022

The conformation of silk fibroin (SF) frozen with polyethylene glycol (PEG) at a molecular weight from 2 kDa to 20 kDa and a mass ratio of PEG:SF from 1:5 to 10:1 was studied by spectral and microscopic methods. It is found that the conformation transition of SF from random coil to β-sheet could be induced by the stress resulting from PEG crystallization at -20℃, and greatly depended on the cooling rate, PEG:SF mass ratio and PEG molecular weight. These findings provide a new method for the preparation of desired SF nanofibers.
- Silk fibroin,
- Polyethylene glycol,
- Freezing,
- Phase separation,
- Crystallization
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