Citation: Jie Chen, Zi-xue Jiao, Lin Lin, Zhao-pei Guo, Cai-na Xu, Yan-hui Li, Hua-yu Tian, Xue-si Chen. Polylysine-modified Polyethylenimines as siRNA Carriers for Effective Tumor Treatment[J]. Chinese Journal of Polymer Science, ;2015, 33(6): 830-837. doi: 10.1007/s10118-015-1632-0 shu

Polylysine-modified Polyethylenimines as siRNA Carriers for Effective Tumor Treatment

1.
Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Science, Changchun 130022, China
2.
School of Materials Science and Technology, Changchun University of Science and Technology, Changchun 130022, China

Received Date: 26 September 2014
Revised Date: 17 October 2014

Fund Project: This work was financially supported by the National Natural Science Foundation of China (Nos. 51222307, 51303173, 51390480, 21474104 and 51403205), the Ministry of Science and Technology of China (International cooperation and communication program 2011DFR51090), Jilin Province Science and Technology Development Program (Nos. 20120306, 20130521011JH).

Polyethylenimine-poly(L-lysine) (PEI-PLL) copolymer was synthesized via ring-opening polymerization of L-lysine N-carboxyanhydride (Lys(Z)-NCA) initiated by PEI. The complexation of PEI-PLL with siRNA was studied by particle size and zeta potential measurements. The flow cytometric analysis and confocal imaging showed its excellent intracellular trafficking ability. PEI-PLL displayed higher gene silencing efficiency and lower cytotoxicity than commercial PEI-25k in vitro. In the antitumor study, PEI-PLL was further combined with siVEGF and showed obviously tumor inhibition effect for the treatment of CT26 tumor model. Therefore, PEI-PLL is a promising siRNA carrier candidate for further antitumor treatment in vivo.
- Gene carrier,
- Gene silencing,
- Polylysine,
- Tumor treatment,
- VEGF siRNA
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1.
沈阳化工大学材料科学与工程学院沈阳 110142